滇黄芩叶总黄酮的自由基清除及抑酶活性

Radicals Scavenging and Enzymes Inhibitory Activities of Total Flavonoid from Leaves of Scutellaria amoena

从滇黄芩(Scutellaria amoena)叶中提取黄酮类成分,经制备获得滇黄芩叶总黄酮(SALF),检测其含量,并考察了SALF清除自由基及抑制α-葡萄糖苷酶、胰脂肪酶活性的能力。结果显示,SALF中黄酮含量为492.1±15.5 mg/g。SALF清除DPPH自由基(DPPH^(•))、ABTS自由基(ABTS^(•+))、羟基自由基(OH^(•))和一氧化氮自由基(NO^(•))的半清除率(SC_(50))分别为57.58±7.10、20.71±1.63、44.40±2.74、104.61±9.39μg/mL。对酿酒酵母α-葡萄糖苷酶(α-glucosidase-Y)活性的半抑制浓度(IC_(50))为228.6±4.61μg/mL,对大鼠肠道α-葡萄糖苷酶(α-glucosidase-R)活性的半抑制浓度为128.4±5.83μg/mL;对胰脂肪酶活性的半抑制浓度为22.41±1.13μg/mL。统计分析表明,SALF具有一定的自由基清除和α-葡萄糖苷酶、胰脂肪酶活性的抑制能力。

The total flavonoid from leaves of Scutellaria amoena(SALF)was obtained and the content was determined.The radicals scavenging,α-glucosidase and pancreatic lipase inhibitory activities of SALF were investigated.The results showed that the content of total flavonoid was 492.1±15.5 mg/g by spectrophotometry with rutin as a reference.The 50%scavenging concentrations(SC_(50))of SALF on DPPH radicals(DPPH^(•)),ABTS radicals(ABTS^(•+)),hydroxyl radical(OH^(•))and nitric oxide radical(NO^(•))were 57.58±7.10,20.71±1.63,44.40±2.74,and 104.61±9.39μg/mL,respectively.The 50%inhibitory concentrations of enzymes activities(IC_(50))of SALF onα-glucosidase from Saccharomyces cerevisiae(α-glucosidase-Y),α-glucosidase from rats small intestine(α-glucosidase-R),and pancreatic lipase were 228.6±4.61,128.4±5.83,and 22.41±1.13μg/mL,respectively.The results after statistical analyzed indicate that SALF has a certain degree of radicals scavenging,α-glucosidase and pancreatic lipase inhibitory activities.