5个SNP突变位点对乌珠穆沁羊不同时期体重的影响

Effects of 5 SNP mutation sites on the body weight of Ujimqin sheep at different time points

绵羊的体重大小能直接反映羊只的生长速度、肥育速度,也是衡量肉羊生长性能的重要指标.本研究以乌珠穆沁羊为研究对象.挑选出100个与绵羊体型相关的SNP位点,同时采集252只乌珠穆沁羊的血液和不同时期体重数据,使用MassARRAY基因分型技术对这些绵羊在选定的SNP位点进行基因分型,并将体重数据与基因分型结果进行关联分析.结果表明,本研究共验证出5个突变位点与乌珠穆沁羊不同时期体重显著相关.进一步对3个错义突变位点进行蛋白结构预测分析发现,3个突变位点CDS序列改变导致其蛋白质三级结构发生不同程度改变,进而导致个体体重差异.本研究旨在挖掘与乌珠穆沁羊生长性状相关的特征位点,为乌珠穆沁羊今后的精准选育提供参考,以期推动乌珠穆沁羊产业的发展.

The weight of sheep can directly reflect their growth and fattening speed and is also an important indicator for measuring the growth performance of meat sheep.This study focused on the Ujimqin sheep as the research object.One hundred SNP loci related to sheep body size were selected,and blood and weight data were collected from 252 Ujimqin sheep at different stages.MassARRAY genotyping technology was used to genotype these sheep strains at the selected SNP loci,and correlation analysis between weight data and genotyping results revealed that a total of 5 mutation sites were significantly correlated with the weight of Ujimqin sheep at different stages.Further protein structure prediction analysis of three missense mutation sites revealed that changes in the CDS of the three mutation sites resulted in varying degrees of changes in their protein tertiary structure,leading to individual weight differences.The aim of this study was to explore the characteristic loci related to the growth traits of Ujimqin sheep,providing a reference for the precise breeding of Ujimqin sheep in the future to promote the development of the Ujimqin sheep industry.