巴戟天多糖的体外抗氧化活性及其对黄酮类化合物溶解性和稳定性的影响

In vitro antioxidant activity of Morinda officinalis polysaccharides and its effect on solubility and stability of flavonoids

目的:探究巴戟天多糖的自身性质以及对黄酮类化合物溶解性和稳定性的影响,以期为巴戟天多糖在中药多组分配伍领域的应用提供有益思路。方法:首先,对巴戟天多糖的含量、p H值、蛋白质含量和分子量进行测定。其次,通过体外试验评估巴戟天多糖对DPPH自由基和羟基自由基的清除能力。最后,考察了巴戟天多糖对杜仲中4种常见的溶解性较差的黄酮单体成分(槲皮素、芦丁、木犀草素和木犀草苷)溶解性和稳定性的影响,并利用相溶解度法研究了二者之间的相互作用机制。结果:巴戟天多糖呈酸性,分子量为1.97×10^(3) Da,对DPPH自由基和羟基自由基具有一定的清除能力,可以与4种黄酮成分形成1∶1包合物,具有一定的增溶促稳作用。高温条件下,巴戟天多糖对木犀草素和木犀草苷均具有显著的促稳作用;在不同p H值条件下,其对槲皮素和木犀草素具有一定的促稳作用,而在碱性条件下对木犀草苷的促稳作用较为显著。结论:本研究为巴戟天和杜仲的合理配伍提供理论依据。

Objective:To explore the properties of Morinda officinalis polysaccharides and the effect on the solubility and stability of flavonoids in order to provide beneficial idea for the application of Morinda officinalis polysaccharides in the field of multi-component compatibility of traditional Chinese medicine.Methods:Firstly,the testing on the content,p H,protein content and molecular weight of Morinda officinalis polysaccharides was carried on.Secondly,the scavenging ability of Morinda officinalis polysaccharides on DPPH radicals and hydroxyl radicals was evaluated by in vitro experiments.Finally,the effects of Morinda officinalis polysaccharides on the solubility and stability of four common flavonoid monomer components with poor solubleness(quercetin,rutin,luteolin and luteoloside)in Eucommia ulmoides were investigated,and the interaction mechanism between them was investigated by using the phase solubility method.Results:Morinda officinalis polysaccharide is acidic,with a molecular weight of 1.97×10^(3) Da.It has a certain ability to remove DPPH free radicals and hydroxyl radicals.It can form a1∶1 inclusion with 4 flavonoid components,which has a certain solubilization and stabilization effect.Under high temperature conditions,Morinda officinalis polysaccharide has a significant effect on promoting the stability of luteolin and luteoloside;under different p H conditions,it has a certain stabilizing effect on quercetin and luteolin,and it has a more significant stabilizing effect on luteoloside under alkaline conditions.Conclusion:This study provides a theoretical basis for the reasonable compatibility of Morinda officinalis and Eucommia ulmoides.