经典名方小承气汤对胃黏膜的保护作用及机制研究

Study on the protective effect and mechanism of the classic formula Xiaochengqi Decoction on gastric mucosa

目的:探究小承气汤的胃黏膜保护作用,并阐明该作用的潜在机制。方法:高效液相色谱法分析小承气汤中各成分及色谱峰归属;MTT法和AnnexinV-PI双染色法用于评价体外小承气汤对细胞增殖和凋亡的作用;氧化因子测定考察小承气汤的抗氧化效果;动物分组为正常组、模型组、低剂量治疗组、高剂量治疗组、奥美拉唑治疗组;通过组织学分析、血清中相关因子分析和Western Blot检测研究小承气汤在体内保护胃黏膜的潜在机制。结果:小承气汤可促进细胞增殖,0.7 mg/mL效果最好;能降低GES-1细胞中ROS、MDA、GSH,提高SOD水平以减轻H2O2诱导的GES-1细胞损伤;小承气汤减轻无水乙醇诱导大鼠胃黏膜损伤,损伤抑制率为94.32%。此外,小承气汤还上调了ZO-1、Occluding蛋白表达,并抑制NF-κB信号通路相关蛋白。结论:小承气汤可以通过减轻氧化应激及炎症反应,抑制NF-κB信号通路,增强紧密连接结构起到促进细胞增殖及减少细胞凋亡作用,进而发挥对胃黏膜的保护作用。

Objective:To explore the protective effect of Xiaochengqi Decoction(XCQD)on injured gastric mucosa in vitro and in vivo,and elucidate the potential mechanism.Methods:HPLC was used to define the components in XCQD.MTT method and Annexin V-PI double staining method were used to evaluate the protective effect of XCQD on oxidative damage cells in vitro.To evaluate the antioxidant effect of XCQD in vitro,the contents of SOD,GSH,and MDA were determined.In vivo study,SD rats were randomly divided into five groups:normal group,model group,low dose treatment group,high dose treatment group,and positive drug group.Histological analysis,analysis of inflammatory and oxidative factors in serum,and Western Blot were used to investigate the potential mechanism of XCQD on protecting acute gastric mucosal injury in vivo.Results:XCQD promotes cell proliferation,with the best effect observed at 0.7 mg/m L,reduces ROS,MDA,GSH in GES-1 cells,increases SOD levels to alleviate the damage of GES-1 cells induced by H_2O_2,and alleviates damage to gastric mucosal in rats caused by anhydrous ethanol,with an inhibition rate of 94.32%.In addition,XCQD also upregulated the expression of ZO-1 and Occludin and inhibited the NF-κB signaling pathway.Conclusion:XCQD can promote cell proliferation and reduce apoptosis by reducing oxidative stress and inflammatory response,inhibiting NF-κB signaling pathway and enhancing the tight junction structure,thereby exerting a protective effect on the gastric mucosa.