补肾壮筋汤多种成分的定量和定性分析

Quantitative and qualitative analysis of various components of Bushen Zhuangjin Decoction

目的:建立高效液相色谱法(HPLC)对补肾壮筋汤多种活性成分进行定性定量分析。方法:冻干粉制备:制备补肾壮筋汤水提液,减压旋转浓缩,制备冻干粉。HPLC法:分析采用Agilent EC-C_(18)(4.6 mm×250 mm,5μm)色谱柱;流动相为水-乙腈,梯度洗脱;检测波长为212 nm(梓醇)、230 nm(松脂醇二葡萄糖苷)和240 nm(莫诺苷、芍药苷、橙皮苷),流速为1 mL/min,柱温为30℃。结果:梓醇、莫诺苷、松脂醇二葡萄糖苷、芍药苷和橙皮苷线性关系良好,R^(2)均>0.9800,平均加样回收率分别为87.63%、94.11%、89.49%、86.80%、110.34%。1 g补肾壮筋汤中药复方中,梓醇含量为5.0010 mg,莫诺苷含量为1.0340 mg,松脂醇二葡萄糖苷含量为0.0772 mg,芍药苷含量为0.4224 mg,橙皮苷含量为4.5980 mg。结论:该方法可行性好、灵敏度高,适用于补肾壮筋汤的质量控制。

Objective:High performance liquid chromatography(HPLC)method was established for the qualitative and quantitative analysis of various active ingredients in Bushen Zhuangjin Decoction.Methods:Preparation of lyophilized powder:the water extract of the Bushen Zhuangjin Decoction was prepared and concentrated under reduced pressure to prepare lyophilized powder.HPLC analysis was performed on an Agilent EC-C_(18)(4.6 mm×250 mm,5μm)column.The mobile phase was wateracetonitrile with gradient elution.The detection wavelengths were 212 nm(catalpol),230 nm(pinoresinol diglucoside)and 240 nm(monoside,paeoniflorin,and hesperidin).The flow rate was 1 mL/min,and the column temperature was 30℃.Results:The linear relationship of catalpa,morroniside,pinoresinol diglucoside,paeoniflorin,and hesperidin was good,R^(2)≥0.9800,and the average recovery rates were 87.63%,94.11%,89.49%,86.80%,110.34%,respectively.In 1 g of Bushen Zhuangjin Decoction,the content of catalpa was 5.0010 mg,the content of monoside was 1.0340 mg,the content of pinerinol diglucoside was0.0772 mg,the content of paeoniflorin was 0.4224 mg,and the content of hesperidin was 4.5980 mg.Conclusion:The method is feasible,sensitive,and suitable for the quality control of Bushen Zhuangjin Decoction.