3种不同海拔裂腹鱼肾脏转录组比较

Transcriptome comparative analysis of kidney tissues of three plateau Schizothoracinae fish species at different altitudes

裂腹鱼亚科鱼类长期生活在青藏高原缺氧环境,是研究低氧适应机制的天然生物模型。对3种裂腹鱼:高海拔(>3600 m)的孤唇裂腹鱼和巨须裂腹鱼与低海拔(<700 m)齐口裂腹鱼的主要渗透调节器官肾脏进行转录组分析。通过对孤唇裂腹鱼和巨须裂腹鱼与齐口裂腹鱼的肾脏转录组进行比较分析,分别筛选出504个和478个差异表达基因,其中有199个共同差异表达基因。对共同差异基因进行GO(Gene Ontology)和KEGG(Kyoto Encyclopedia of Genes and Genomes)的富集分析发现,两组共同差异基因主要富集在糖酵解/糖原生成过程、脂质生物合成过程和血管生成等通路上。其中eno3、fbp1α、prpf19和ctsz等基因的高表达可能在高海拔裂腹鱼长期适应低氧环境中起着重要作用。本研究为揭示高原鱼类长期低氧适应提供了新思路。

Subfamily has lived in the anoxic environment of Qinghai-Tibet Plateau for a long time,which is a natural biological model to study the adaptation mechanism of hypoxia.Transcriptome analysis was made on the kidneys of three species of Schizothorax curvilabiatus and Schizothorax macropogon at high altitude(>3600 m)and Schizothorax prenanti at low altitude(<700 m).By comparing and analyzing the renal transcriptome of Schizothorax unicornis,Schizothorax megacephalus and Schizothorax homostoma,504 and 478 differentially expressing genes were screened out respectively,among which 199 genes were commonly differentially expressed.The enrichment analysis of common differential genes by GO(Gene Ontology)and KEGG(Kyoto encyclopedia of genes and genomes)showed that the two groups of common differential genes were mainly enriched in glycolysis/glycogen production,lipid biosynthesis,and angiogenesis.Among them,the high expression of eno3,fbp1α,prpf19,and ctsz may play an important role in the long-term adaptation of Schizothorax at high altitude to hypoxic environment.This study provides a new idea for revealing the longterm hypoxia adaptation of plateau fish.