抑制Wnt/β-catenin信号通路对始初小头虫再生的影响

Experiment study on the effect of inhibition of Wnt/β-catenin signaling on the regeneration of Capitella teleta

Wnt/β-catenin信号通路在涡虫等多种生物的再生中发挥重要作用,本研究以环节动物始初小头虫(Capitella teleta)为研究对象,初步探究Wnt/β-catenin信号通路对小头虫再生的影响。将截断损伤后的样本持续浸泡于30μmol/L浓度外源性Wnt/β-catenin信号通路小分子化合物抑制剂XAV-939中,发现小头虫的再生受到了抑制,与对照组相比再生速度明显减慢,且不能形成正常的再生芽基组织,通过神经和肌肉标记发现Wnt信号通路被抑制后,小头虫再生过程中的神经和肌肉生长也受到了影响。后续通过EdU(5-Ethynyl-2’-deoxyuridine)标记发现抑制剂处理组样本的增殖细胞数量显著少于正常组样本,表明Wnt/β-catenin信号通路被抑制后会影响小头虫再生过程中的增殖细胞产生,从而调控小头虫的再生过程。在几种典型物种中Wnt通路调控再生的比较研究表明Wnt信号通路在刺胞动物和冠轮动物全身再生中的功能相对保守。

Wnt/β-catenin signaling plays an important role in regeneration in multiple species such as planaria.The study aimed to investigate the effect of Wnt/β-catenin signaling on the regeneration in the annelid,Capitella teleta.Sustained incubation of samples after amputation in 30μmol/L XAV-939,an exogenous Wnt/β-catenin signaling small molecular inhibitor,revealed that the regeneration of C.teleta was inhibited,with significantly slower regeneration compared to that in the control group and failure to form a regeneration blastema.Neural and muscle growth during regeneration were also affected by inhibition of Wnt signaling revealed by neural and muscle labeling.Subsequently,EdU labeling indicated that the number of proliferating cells in the XAV 939-treated group was significantly less than that in the control group,suggesting that the suppression of the Wnt/β-catenin signaling may have affected the proliferating cells during the regeneration of C.teleta,which also further demonstrating that the Wnt/β-catenin signaling may be involved in the regeneration of C.teleta,and play an important role in the early stage of regeneration.Comparative studies on the regulation of regeneration by wnt signaling in several typical species have shown that the Wnt signaling is relatively conserved in the whole-body regeneration of Cnidaria and Lophotrochozoa.