具有宽带激励功能的叶绿素荧光仪的开发研究

Research and Development of Chlorophyll Fluorescence Meter with Broadband Excitation Function

叶绿素荧光检测技术作为一种无损检测技术应用十分广泛。传统叶绿素荧光检测技术激发信号时使用的阶跃或调制脉冲(PAM)频带窄,而光合系统是一个高阶的宽带系统,难以激发出光合系统所有的动态特性,使叶绿素荧光信号含有的信息丰富度受限。目前市面上的叶绿素荧光仪均不具备宽带激励功能,限制了新兴的人工智能算法能够处理复杂信号、挖掘丰富信息的能力。针对该问题,基于伪随机二进制序列(PRBS)信号,设计开发了一套具有宽带激励功能的叶绿素荧光仪。该仪器同时能测量传统的叶绿素荧光诱导动力学曲线(OJIP)与PAM曲线,对5种不同植物在3种激发光下的叶绿素荧光的信息熵进行对比,结果表明,PRBS激发的叶绿素荧光具有最高的信息熵。该仪器能够提供信息更丰富的叶绿素荧光信号,有望为植物生理及环境胁迫检测提供新型的科学仪器。

Chlorophyll fluorescence detection technology is widely used as a nondestructive detection method;however,the frequency band of the step pulse or the modulated pulse(PAM)used in the traditional chlorophyll fluorescence detection technology to excite signals is narrow,and a photosynthetic system is a highorder broadband system,which is difficult to excite all the dynamic characteristics.These limit the information richness contained in chlorophyll fluorescence signals.Currently available commercial chlorophyll fluorescence meters do not contain broadband excitation.The absence of this function limits the ability of emerging artificial intelligence algorithms to process complex signals for mining rich information.Thus,in this study,we developed a chlorophyll fluorescence instrument with a broadband excitation function based on the pseudorandom binary sequence(PRBS)signal.The developed instrument can measure the traditional chlorophyll fluorescence induction OJIP and PAM kinetics.The information entropy of chlorophyll fluorescence of five different plants under three different light sources confirmed that the chlorophyll fluorescence excited by PRBS has the highest information entropy.In addition,the instrument can provide chlorophyll fluorescence signals with more information and is expected to contribute to a new scientific instrument for detecting plant physiology and environmental stress.