不同干燥处理的树参叶黄酮类成分的非靶向代谢组学分析

Analysis of the Difference of Flavonoids in Dendropanax dentiger Leaves with Different Drying Processes Based on Untargeted Metabolomics

运用超高效液相色谱串联质谱技术对3种(真空冷冻干燥、热风干燥和微波干燥)干燥方式下的树参(Dendropanax dentiger)叶黄酮物质进行检测,使用主成分分析和正交偏最小二乘判别分析法鉴定差异代谢物。共鉴定到204个黄酮化合物,以黄酮(53个)、黄酮醇(37个)和异黄酮(24个)化合物为主;筛选到80个差异化合物,其中热风干燥与真空冷冻干燥有69个差异物,热风干燥与微波干燥具有67个差异物,微波干燥与真空冷冻干燥有45个差异物。这些差异代谢物主要参与次生代谢产物的生物合成,且对表征不同干燥方式的样品贡献率明显。通过差异代谢物的上调或下调研究发现,真空冷冻干燥加工方式能较好地保留树参叶中的黄酮成分,其次为微波干燥。

Ultrahigh performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was used to detect the flavonoids accumulated in leaves of Dendropanax dentiger under three drying methods(freeze-drying,hot-air drying and microwave drying).Principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA)were applied to identify the differential metabolites.The results indicated that 204 flavonoid compounds were identified,dominated by 53 flavonoids,37 flavonols and 24 isoflavones.A total of 80 differential compounds were screened,among which 69 different metabolites were found between hot-air drying and freeze-drying,67 were found between hot-air drying and microwave drying,and 45 were found between microwave drying and freeze-drying.The metabolites of these differences were mainly involved in the biosynthesis of secondary metabolites.The contribution rate of different substances to the characterization of different drying methods was obvious.Through the study of up-regulation and down-regulation of differential metabolites,it was found that the flavonoid components in the leaves could be retained better by freeze-drying,followed by microwave drving.