中药金属配合物超分子水凝胶的制备及其多重生物活性评价

Preparation of traditional Chinese medicine metal complexes supramolecular hydrogel and its multiple biological activity evaluation

本研究以中药活性成分甘草酸与锌离子形成金属配合物超分子水凝胶为例,探究不同浓度锌离子对金属配合物自组装的影响,并对形成的无载体超分子水凝胶的性质及组装机制进行表征;采用扫描电子显微镜和zeta电位表征了甘草酸-锌离子配合物水凝胶的微观形貌及稳定性,其微观形态为1μm左右的类球形颗粒,且长期放置稳定性良好;采用流变仪检测其材料学性质,其具有优异的机械稳定性、可愈合性和可逆性;通过体外抑菌实验,发现甘草酸-锌离子配合物无载体超分子水凝胶相比甘草酸具有增强的抑菌效果;分别采用脂多糖和过氧化氢诱导RAW 264.7细胞损伤模型进行活性评价,发现该水凝胶同时具有抗炎和抗氧化的功效。以上研究结果表明,甘草酸-锌离子金属配合物水凝胶不仅具有良好的材料学性质,自身还具有良好的抗菌、抗炎、抗氧化多重生物活性,具有作为无载体多功能抗菌敷料的潜在临床研究价值,本研究为从天然中药活性成分中发现新型生物医疗材料提供参考。

The effect of different concentrations of glycyrrhizic acid(GA)and Zn2+on the self-assembly of metal complexes was investigated by forming metal complexes,and the properties and assembly mechanisms of the formed carrier-free supramolecular hydrogel were characterised.Scanning electron microscopy(SEM)and zeta potential were used to characterise the microscopic morphology and stability of the GA-Zn complex hydrogel,which had spherical-like particles of about 1μm with good stability;the rheometer was used to detect its materialistic properties,which showed excellent stability,self-healing property and reversibility;through in vitro bacterial inhibition,it was found that the GA-Zn carrier-free supramolecular hydrogel has enhanced bacterial inhibition function after assembly.The hydrogel was also found to possess both anti-inflammatory and antioxidant efficacy when evaluated using LPS and H2O2 induced RAW 264.7 cell damage models,respectively.The above results suggest that GA-Zn hydrogel not only has good materialistic properties,but also possesses good antibacterial,anti-inflammatory and antioxidant activities,which has the value of clinical research as a carrier-free multi-functional antimicrobial dressing,and the present study provides a reference for the discovery of novel biomedical materials from active molecules of natural traditional Chinese medicine.