木瓣树中3个新的倍半萜类化合物及其法尼醇X受体激活作用研究

Three new sesquiterpenes from Xylopia vielana Pierre and their effects of farnesoid X receptor activation

采用硅胶、ODS和半制备HPLC等多种色谱学技术从木瓣树枝叶中分离得到了11个化合物。化学结构经高分辨质谱、核磁共振谱、ECD等多种波谱分析方法,鉴定为:vielana A (1)、vielana B (2)、vielana C (3)、10-oxoisodauc-3-en-15-al (4)、1α-hydroxyisodauc-4-en-15-al (5)、mokko lactone (6)、11β,13-dihydrocostunolide (7)、eurylosesquiterpenol E (8)、epi-α-cadinol (9)、mustakone (10)、7-epi-amiteo (11)。化合物1~3为新化合物,其余化合物均首次从木瓣树中分离得到。化合物1和2增加了法尼醇X受体(farnesoid X receptor,FXR)下游靶基因BSEP启动子的转录活性,表明其具有潜在的FXR激活作用。

Eleven compounds were isolated from the twigs and leaves of Xylopia vielana Pierre by various chromatographic techniques such as silica gel,ODS and the semi-preparative HPLC.Their chemical structures were identified by HR-ESI-MS,NMR,ECD and other spectroscopic methods as vielana A(1),vielana B(2),vielana C(3),10-oxo-isodauc-3-en-15-al(4),1α-hydroxyisodauc-4-en-15-al(5),mokko lactone(6),11β,13-dihydrocostunolide(7),eurylosesquiterpenol E(8),epi-α-cadinol(9),mustakone(10),7-epi-amiteo(11).Among them,compounds 1-3 were new compounds,and the rest compounds were isolated from this plant for the first time.Compounds 1 and 2 increased the transcriptional activity of the farnesoid X receptor(FXR) downstream target gene BSEP promoter,indicating that they have potential of FXR activation.