基于UPLC-MS/MS法比较不同产地布渣叶中19个成分含量

Determination of 19 components in Microctis Folium from different production areas based on UPLC-MS/MS

基于UPLC-MS/MS法同时测定不同产地布渣叶中19个化合物的含量。以50%甲醇为提取溶剂,采用Agilent ZORBAX SB C18 (150 mm×2.1 mm,1.8μm)色谱柱;以乙腈-0.1%乙酸为流动相进行梯度洗脱,流速为0.3 m L·min^(-1),柱温30℃,进样量为2μL;采用电喷雾离子源,在负离子模式下进行检测。结果表明,建立的UPLC-MS/MS方法可较好地分离19个成分,19个成分的方法学考察结果良好。通过正交偏最小二乘法-判别分析(OPLS-DA)可将28批不同产地布渣叶样品分为3类,广东、广西、海南各自归为一类,并筛选出影响不同产地质量差异的10个标志性化合物。本实验所建立的方法准确、可靠、灵敏度高、重复性好,可为布渣叶质量标准的建立及后续深入完善其质量控制体系、临床安全用药等相关研究提供依据。

The paper is to establish an UPLC-MS/MS method for the simultaneous determination of 19components in Microctis Folium from different production areas.The 50%methanol was used as extraction solvent.The Agilent ZORBAX SB C18 (150 mm×2.1 mm,1.8μm) column was used;mobile phase was acetonitrile-0.1%acetic acid with gradient elution,flow rate was 0.3 m L·min~(-1),colume temperature was 30℃,and the injection volume was 2μL;electrospray ionizaton source was used and detected in negative ion mode.The results showed that the established UPLC-MS/MS method could well separate the 19 components,and the methodological investigation results of 19 components were good.By means of orthogonal partial least squares discriminant analysis (OPLS-DA),28 batches of Microctis Folium samples from different production areas can be divided into three categories,Guangdong,Guangxi and Hainan are each classified into one category,and 10 signature compounds which affecting the quality differences of different production areas were screened out.The established method is accurate,reliable,sensitive and reproducible.It can provide a basis for the establishment of the quality standard of Microctis Folium,as well as for safety and quality research.