吐鲁番葡萄病毒病的发生情况及病原鉴定

Occurrence and pathogen identification of grape virus disease in Turpan

为明确吐鲁番葡萄病毒病发生情况及感染病毒种类,对吐鲁番葡萄种植区的葡萄病毒侵染情况进行调查,依据高通量测序结果,基于田间调查结合RT-PCR技术对采集的疑似葡萄病毒样本进行检测。结果表明,吐鲁番葡萄病毒病的症状一般在6-7月份左右出现;采集的183份样品中共检测到5种病毒,分别为葡萄克孜尔萨帕克病毒(GKSV)、葡萄卷叶相关病毒4(GLRaV-4)、葡萄浆果内坏死病毒(GINV)、葡萄蚕豆萎蔫病毒(GFabV)、沙地葡萄茎痘相关病毒(GRSPaV),总检出率分别为8.20%、30.60%、18.58%、10.92%、25.68%,其中,GLRaV-4检出率为最高;葡萄病毒复合侵染发生普遍,有两种或3种复合侵染类型,总检出率分别为27.32%、8.74%。对吐鲁番葡萄病毒病进行调查与鉴定,明确了葡萄病毒种类,掌握了不同葡萄种植区葡萄病毒的发生现状及病毒携带情况,为吐鲁番葡萄病毒病的早期监测和防控提供理论指导。

In order to clarify the occurrence of grapevine virus disease and the types of virus infection in Turpan,the infection of grapevine virus in Turpan grape growing area was investigated.According to high-throughput sequencing results,field investigation combined with RT-PCR technology was used to de-tect suspected grape virus samples.The results showed that the symptoms of Turpan grape virus disease were generally around June to July;A total of 183 samples were collected and five kinds of viruses were detected,including Grapevine Kizil Sapak virus(GKSV),grapevine leafroll-associated virus 4(GLRaV-4),grapevine berry inner necrosis virus(GINV),grapevine fabavirus(GFabV),grapevine rupestris stem pit-ting-associated virus(GRSPaV),The total detection rate were 8.20%,30.60%,18.58%,10.92%,25.68%,and the detection rate of GLRaV-4 was the highest;Multiple infection of grapevine virus was common,there were two or three types of multiple infection,and the total detection rate of 27.32%,8.74%respectively.This study investigated and identified grape virus disease in Turpan,clarified the species of grape virus,grasped the occurrence status and virus carrying situation of grape virus in different grape growing areas,to provide theoretical guidance for the early monitoring and control of grape virus disease in Turpan.