敲除OsLRR-RLK18基因提高水稻对褐飞虱的抗性

Knocking out OsLRR-RLK18 in rice enhances the resistance to brown planthopper Nilaparvata lugens

为明确富亮氨酸重复类受体蛋白激酶(leucine-rich repeat receptor-like kinase,LRR-RLK)编码基因OsLRR-RLK18在调控水稻对褐飞虱Nilaparvata lugens防御中的作用及机理,以野生型水稻品系、OsLRR-RLK18基因敲除纯合水稻品系为研究对象,测定不同水稻品系的株高和根长、不同水稻品系对褐飞虱生物学参数的影响及褐飞虱为害后不同水稻品系中防御信号分子含量和防御化合物含量。结果显示,敲除OsLRR-RLK18基因后水稻株高和根长降低,褐飞虱为害后期诱导的茉莉酸、茉莉酸-异亮氨酸和脱落酸含量显著增加,但水稻组成型(未受褐飞虱为害)和为害早期的水杨酸和过氧化氢含量降低,引起水稻多种挥发物组分释放量增加,木质素、对香豆酰腐胺及樱桃苷、刺苞菊苷和大波斯菊苷3种类黄酮含量下降,最终导致褐飞虱卵孵化率和产卵量显著降低。表明OsLRR-RLK18基因参与了水稻对褐飞虱为害的防御反应,在调控水稻对褐飞虱的抗性中发挥着一定作用。

To elucidate the role of the leucine-rich repeat receptor-like kinase(LRR-RLK)gene OsLRRRLK18 in regulating rice defense responses against the brown planthopper Nilaparvata lugens and its underlying mechanisms,we utilized both the wild-type rice line and OsLRR-RLK18 knockout rice lines as research materials to assess the shoot height and root length of different rice plants,examine the effect on N.lugens biological parameters across different rice lines,and analyze the levels of defenserelated signal molecules and defensive compounds in different rice plants before and after infestation by N.lugens females.The results showed that knocking out OsLRR-RLK18 significantly increased the levels of jasmonic acid,jasmonic acid-isoleucine,and abscisic acid in rice at late stage of N.lugens infestation,but decreased the basal(non-infested)and N.lugens-induced(earlier stage of infestation)levels of salicylic acid and hydrogen peroxide.Moreover,knocking out OsLRR-RLK18 enhanced the emission of multiple volatile compounds from rice plants while reducing the levels of lignin,p-coumaroyl putrescine,and three flavonoids(carlinoside,cosmosiin and prunin).These changes finally led to a significant decrease in the number and hatchability of N.lugens eggs.These results indicate that OsLRR-RLK18 is involved in the defense responses in rice induced by N.lugens infestation,and plays a role in regulating rice the resistance to N.lugens.