基于直接电子转移的柔性纤维葡萄糖传感器

Flexible Fiber Glucose Sensor Based on Direct Electron Transfer

酶基电化学葡萄糖传感器具有优异的选择性和灵敏度,基于直接电子转移原理的第三代传感器有望解决第一、二代传感器所面临的易受氧气浓度干扰、电子介体不稳定等问题.因此,本研究基于聚3,4-乙烯二氧噻吩:聚苯乙烯磺酸盐(PEDOT:PSS)和葡萄糖脱氢酶(GDH)双组分开发了柔性纤维第三代葡萄糖传感器.该纤维传感器在10~500μmol/L范围内具有良好的线性(R^(2)=0.9968),灵敏度可达62.53μA/(mmol·L^(-1)·cm^(2)),且不受氧气影响.纤维传感器还具有良好的柔性和可编织性,将其集成到织物中,实现了人在运动时汗液中葡萄糖的连续实时监测,为制备满足可穿戴需求的第三代葡萄糖传感器提供了新思路.

Real-time monitoring of glucose concentration in vivo holds significant importance for disease diagnosis and treatment.The enzyme-based electrochemical glucose sensor has garnered considerable attention in the realm of real-time glucose monitoring due to its outstanding selectivity and sensitivity.Despite the development of first and second generation electrochemical glucose sensors,they encounter numerous challenges derived from their sensing principles,including interference from oxygen concentration,instability of electronic mediators,and leaching toxicity.The third generation of glucose sensor based on direct electron transfer between enzyme and electrode is anticipated to address these challenges.Here,we report an all-polymer-based flexible fiber glucose sensor(FGS)based on direct electron transfer,leveraging poly(3,4-vinyldioxythiophene):polystyrene sulfonate(PEDOT:PSS)and glucose dehydrogenase(GDH)as its constituents.The FGS exhibits commendable linearity(R^(2)=0.9968)within the range of 10-500μmol/L and demonstrates a sensitivity of 62.53μA/(mmol·L^(-1)·cm^(2)).The FGS is seamlessly integrated into fabric,enabling continuous real-time monitoring of glucose levels in human sweat during exercise.This work presents a novel approach for developing the third-generation glucose sensors to cater to the requirements of wearable technology.