接触辉光放电等离子体对互隔交链孢的灭活机理及其动力学

Inactivation mechanism and kinetic of Alternaria alternata using contact glow discharge plasma

互隔交链孢(Alternaria alternata)是一种引起梨、枸杞等黑霉病最主要的病原菌。本文研究了接触辉光放电等离子体(CGDP)对互隔交链孢的杀菌作用与机理。首先通过考察不同时间、电解质和电压条件对杀菌效率的影响,确定了最佳的杀菌工艺与条件。其次通过评估CGDP对互隔交链孢细胞膜完整性和孢子内核酸和蛋白质的渗漏量等的影响,研究了CGDP对互隔交链孢的杀菌机理,并构建了灭活动力学模型。结果表明,CGDP对互隔交链孢的杀菌效果显著。杀菌率随着电压的升高和处理时间的延长而增大;孢子内核酸和蛋白质的渗漏量随着处理时间的延长逐渐升高。碘化丙啶、二乙酸荧光素染色和扫描电镜结果表明,CGDP对互隔交链孢的杀菌作用是通过CGDP破坏其形状和细胞膜完整性实现的。孢子失活动力学符合Dose-response模型。研究结果为CGDP对互隔交链孢的杀菌机理提供了重要的理论基础。

Alternaria alternata(A.alternata)is a major pathogenic fungus that causes black mold disease in pears,goji berries,and other crops.In this study,we investigated the inactivation mechanism and kinetics of A.alternata by using contact glow discharge plasma(CGDP).First,the optimal sterilization process and conditions were determined by examining the effects of exposure time,electrolytes,and voltage on the fungicidal rate.Second,the sterilization mechanism of A.alternata by CGDP was studied by evaluating cell membrane integrity and leakage of spore nucleic acids and proteins,and an inactivation kinetic model was constructed.These results showed that CGDP had a significant sterilization effect on A.alternata..The sterilization rate increased with increasing voltage and treatment duration.Leakage of spore nucleic acids and proteins gradually increased with increasing treatment time.Propidium iodide and Fluorescein diacetate staining,as well as scanning electron microscopy indicated that the effect of CGDP on A.alternata was due to the disruption of its morphology and cell membrane integrity.Spore inactivation kinetics were consistent with dose-response modeling,and the results provide an important theoretical basis for further studies on the bactericidal mechanism of A.alternata.