辛麻颗粒对慢性哮喘小鼠呼吸道黏膜免疫功能的影响

Effect of Xinma Granules on Immune Function of Respiratory Tract Mucosa in Chronic Asthmatic Mice

【目的】探讨辛麻颗粒对慢性哮喘小鼠呼吸道黏膜免疫功能的影响。【方法】将50只雌性BALB/C小鼠随机分成正常组、模型组、辛麻颗粒低剂量组、辛麻颗粒高剂量组、地塞米松组,每组10只。除正常组,其余各组小鼠采用卵清白蛋白(OVA)致敏和激发法建立慢性哮喘模型。给予相应干预后,采用酶联免疫吸附分析(ELISA)测定支气管灌洗液分泌型免疫球蛋白A(sIgA)、免疫球蛋白E(IgE)水平,苏木素-伊红(HE)染色法观察肺组织病理学改变,Western Blot法检测肺组织E钙黏蛋白(E-cadherin)表达。【结果】HE染色可见哮喘小鼠明显气道炎症。模型组小鼠支气管灌洗液sIgA浓度低于正常组(P<0.05);辛麻颗粒高、低剂量组及地塞米松组sIgA浓度高于模型组(P<0.05)。模型组小鼠支气管灌洗液IgE浓度高于正常组(P<0.05);辛麻颗粒高、低剂量组及地塞米松组IgE浓度低于模型组(P<0.05)。模型组小鼠肺组织E-cadherin蛋白相对表达量低于正常组(P<0.05);辛麻颗粒高、低剂量组及地塞米松组小鼠肺组织中E-cadherin蛋白相对表达量高于模型组(P<0.05)。辛麻颗粒高剂量组和地塞米松组各指标改善效果优于辛麻颗粒低剂量组(P<0.05),且2组比较,差异无统计学意义(P>0.05)。【结论】辛麻颗粒可能通过改善气道炎症、增高呼吸道sIgA浓度、增强呼吸道E-cadherin蛋白的表达,提高哮喘小鼠呼吸道黏膜免疫功能。

To investigate the effect of Xinma Granules on respiratory mucosal immune function in chronic asthmatic mice.Methods Fifty female BALB/C mice were randomly divided into normal group,model group,low-dose Xinma Granules group,high-dose Xinma Granules group and Dexamethasone group,with 10 mice in each group.Except for the normal group,the mice in the other groups were sensitized and challenged with ovalbumin(OVA)to establish a chronic asthma model.After corresponding treatment,the levels of secretory immunoglobulin A(sIgA)and immunoglobulin E(IgE)in bronchial lavage fluid were measured by enzyme-linked immunosorbent assay(ELISA).The pathological changes of lung tissue were observed by hematoxylin-eosin(HE)staining.The expression of E-cadherin in lung tissue was detected by Western Blot.Results HE staining showed obvious airway inflammation in asthmatic mice.The concentration of sIgA in the bronchial lavage fluid of the model group was lower than that of the normal group(P<0.05);the concentration of sIgA in the high-dose and the low-dose of Xinma Granules groups and the Dexamethasone group was higher than that in the model group(P<0.05);the concentration of IgE in bronchial lavage fluid of model group was higher than that of normal group(P<0.05);the concentration of IgE in the high-dose and the low-dose Xinma Granules groups and the Dexamethasone group was lower than that in the model group(P<0.05).The relative expression of E-cadherin protein in lung tissue of the model group was lower than that of the normal group(P<0.05);the relative expression of E-cadherin protein in lung tissue of mice in the high-dose and low-dose Xinma Granules groups and Dexamethasone group was higher than that in model group(P<0.05).The improvement effect on above various indexes in high-dose Xinma Granules group and Dexamethasone group were superior to that in low-dose Xinma Granules group(P<0.05),the differences between the both groups were statistically insignificant(P>0.05).Conclusion Xinma Granules may improve the airway mucosal immune function of asthmatic mice by improving airway inflammation,increasing the concentration of sIgA in the respiratory tract,and enhancing the expression of E-cadherin protein in the respiratory tract.