稽留流产患者绒毛组织miR-19b与PTEN的表达及临床意义

Expression and Clinical Significance of miR-19b and PTEN in Villus Tissue of Patients with Missed Abortion

目的检测稽留流产患者绒毛组织miR-19b与PTEN的表达水平并分析其临床意义。方法选取2022年1月-5月在本院诊断治疗的稽留流产患者30例作为试验组,同期选取30例正常早孕并要求终止妊娠妇女为对照组,比较两组绒毛组织中miR-19b、PTEN的表达水平及相关性。结果试验组miR-19b相对表达量(1.792±0.905)高于对照组(0.538±0.443),PTEN mRNA相对表达量(1.909±1.099)低于对照组(6.316±2.597),PTEN蛋白相对表达量(0.036±0.016)低于对照组(0.084±0.013)(P<0.05)。相关性分析显示,对照组绒毛组织中miR-19b与PTEN的表达呈负相关(r=-0.584,P=0.001)。结论稽留流产患者绒毛组织miR-19b表达升高,可能通过调控PTEN影响滋养细胞的增殖、凋亡及侵袭。

Objective To detect the expression levels of miR-19 b and PTEN in villus tissues of patients with missed abortion and analyze their clinical significance.Methods A total of 30 patients with missed abortion diagnosed and treated in our hospital from January to May 2022 were selected as the experimental group,and 30 normal early pregnant women who required termination of pregnancy were selected as the control group.The expression levels and correlation of miR-19b and PTEN in villus tissues of the two groups were compared.Results The relative expression of miR-19b was(1.792±0.905)in the experimental group,which was higher than(0.538±0.443)in the control group;the relative expression of PTEN mRNA was(1.909±1.099)in the experimental group,which was lower than(6.316±2.597)in the control group;and the relative expression of PTEN protein was(0.036±0.016)in the experimental group,which was lower than(0.084±0.013)in the control group(P<0.05).Correlation analysis showed that there was a negative correlation between the expression of miR-19b and PTEN in villus tissues of control group(r=-0.584,P=0.001).Conclusion The expression of miR-19b in villus tissue of patients with missed abortion is increased,which may affect the proliferation,apoptosis and invasion of trophoblast cells by regulating PTEN.