急性痛风性关节炎患者外周血黑色素瘤缺乏因子2信号通路相关细胞因子及焦孔素D表达变化观察

Expression changes of AIM2 signal pathway-related cytokines and GSDMD in peripheral blood of patients with acute gouty arthritis

目的观察急性痛风性关节炎患者外周血黑色素瘤缺乏因子2(AIM2)炎症小体信号通路相关细胞因子[AIM2、双链DNA(dsDNA)、IL-1β、IL-18]及焦孔素D表达变化,并分析单个核细胞中AIM2、焦孔素D与急性痛风性关节炎患者病情[WBC、PLT、ESR、CRP、IL-1β、IL-18、BMI、UA、BUN、肌酐(SCr)、肌酐清除率(CCr)、TG、TC]的关系,以探讨AIM2炎症小体信号通路介导的细胞焦亡在急性痛风性关节炎发生、发展中的作用。方法纳入30例急性痛风性关节炎患者(观察组)和30例健康对照者(对照组),比较两组外周血单个核细胞(PBMCs)中AIM2、焦孔素D及血清中IL-1β、IL-18、双链DNA(dsDNA)水平。采用Spearman相关分析AIM2 mRNA和焦孔素D mRNA与急性痛风性关节炎患者病情相关指标(IL-1β、IL-18、WBC、PLT、ESR、CRP、BMI、UA、BUN、SCr、CCr、TG、TC)的关系。结果与对照组比较,观察组PBMCs中AIM2和焦孔素D mRNA的表达水平高(P均<0.05),血清dsDNA、IL-1β、IL-18水平高(P均<0.01)。PBMCs中AIM2 mRNA与WBC、ESR、CRP、BMI、TC、TG、sCr正相关(P均<0.05),焦孔素D mRNA与ESR、IL-1β、sCr正相关(P均<0.05)。结论急性痛风性关节炎患者外周血PBMCs中AIM2、焦孔素D及血清中dsDNA、IL-1β、IL-18水平均升高,AIM2炎症小体介导的细胞焦亡可能参与了急性痛风性关节炎的发生、发展。

Objective To observe the changes in the expression of absent in melanoma 2(AIM2),inflammasome signal pathway-related factors,double-stranded DNA(dsDNA),interleukin(IL)-1β,IL-18 and gasdermin D(GSDMD)in peripheral blood mononuclear cells(PBMCs)of patients with acute gouty arthritis,to analyze the relationship between AIM2,GSDMD in mononuclear cells and the condition of patients with acute gouty arthritis[WBC,PLT,ESR,CRP,IL1β,IL-18,BMI,UA,BUN,serum creatinine(SCr),creatinine clearance rate(CCr),TG,TC],and to explore the role of AIM2 inflammasome signal pathway-mediated pyroptosis in the development and progression of acute gouty arthritis.Methods Thirty patients with acute gouty arthritis(observation group)and 30 healthy controls(control group)were included to compare the levels of AIM2,GSDMD in PBMCs,and IL-1β,IL-18,and dsDNA in serum.Spearman correlation analysis was used to investigate the relationships between AIM2 mRNA,GSDMD mRNA,and the related indicators of patients with acute gouty arthritis(IL-1β,IL-18,WBC,PLT,ESR,CRP,BMI,UA,BUN,SCr,CCr,TG,and TC).Results Compared with the control group,the expression levels of AIM2 and GSDMD mRNA in PBMCs of the observation group were higher(both P<0.05),and the levels of serum dsDNA,IL-1β,IL-18 were higher(all P<0.01).AIM2 mRNA in PBMCs was positively correlated with WBC,ESR,CRP,BMI,TC,TG,and SCr(all P<0.05),and GSDMD mRNA was positively correlated with ESR,IL-1β,and SCr(all P<0.05).Conclusion The levels of AIM2,GSDMD in PBMCs,and dsDNA,IL-1β,and IL-18 in the serum of patients with acute gouty arthritis increase,indicating that AIM2 inflammasome-mediated pyroptosis may be involved in the development and progression of acute gouty arthritis.