非小细胞肺癌组织AIP1、EDIL3表达变化观察

Expression changes of AIP1 and EDIL3 in non-small-cell lung cancer tissues

目的观察非小细胞肺癌(NSCLC)组织凋亡信号调节激酶1-相互作用蛋白1(AIP1)、表皮生长因子样结构域3(EDIL3)表达变化,分析其与微血管密度(MVD)、临床病理特征及预后的关系,为NSCLC的靶向治疗提供新的干预靶点。方法纳入155例NSCLC患者,免疫组织化学法检测癌组织和癌旁组织中AIP1、EDIL3蛋白,Weidner法检测癌组织MVD。比较不同AIP1、EDIL3表达的NSCLC患者MVD值及不同临床病理特征NSCLC患者癌组织AIP1、EDIL3表达水平。Kaplan-Meier生存曲线分析不同AIP1、EDIL3表达的NSCLC患者生存情况,多因素Cox回归分析NSCLC患者预后的影响因素。结果与癌旁组织比较,NSCLC癌组织中EDIL3阳性表达率高、AIP1阳性表达率低(P均<0.05)。不同分化程度、淋巴结转移、肿瘤直径、TNM分期的NSCLC患者癌组织中AIP1、EDIL3蛋白阳性表达率比较,P均<0.05。AIP1阳性表达的NSCLC患者癌组织MVD值低于AIP1阴性表达者(P<0.05),EDIL3阳性表达的NSCLC患者癌组织MVD值高于EDIL3阴性表达者(P<0.05)。EDIL3阳性表达的NSCLC患者3年总生存(OS)率低于EDIL3阴性表达者(P<0.05),AIP1阳性表达的NSCLC患者3年OS率高于AIP1阴性表达者(P<0.05)。淋巴结转移、TNM分期ⅢA期、EDIL3阳性表达是NSCLC患者预后不良的危险因素(P均<0.05),AIP1阳性表达是保护因素(P<0.05)。结论NSCLC癌组织中AIP1阳性表达率降低,EDIL3阳性表达率升高;癌组织中AIP1、EDIL3阳性表达率与MVD、分化程度、淋巴结转移、肿瘤直径、TNM分期有关,是NSCLC预后不良的影响因素。

Objective To investigate the changes in the expression levels of apoptosis signal-regulating kinase 1-interacting protein 1(AIP1)and epidermal growth factor-like repeats and discoidin I-like domains 3(EDIL3)in non-smallcell lung cancer(NSCLC)tissues,and to analyze their relationships with microvascular density(MVD),clinical and pathological features,and prognosis in order to provide new intervention targets for targeted treatment of NSCLC.Methods Totally 155 cases of NSCLC patients were included,and the AIP1 and EDIL3 proteins in cancer tissues and adjacent cancer tissues were detected by immunohistochemical method,and the MVD in cancer tissues was detected by Weidner method.We compared the MVD values of NSCLC patients with different AIP1 and EDIL3 expression and the expression levels of AIP1 and EDIL3 in the cancer tissues of NSCLC patients with different clinical and pathological characteristics.The survival situation of NSCLC patients with different AIP1 and EDIL3 expression levels was analyzed by Kaplan-Meier survival curve,and the influencing factors for the prognosis of NSCLC patients were analyzed by multivariate Cox regression.Re⁃sults Compared with the adjacent tissues,the positive expression rate of EDIL3 was higher and the positive expression rate of AIP1 was lower in the cancer tissues(both P<0.05).Significant differences were found in the positive expression rates of AIP1 and EDIL3 proteins in cancer tissues of NSCLC patients with different degrees of differentiation,lymph node metastasis,tumor diameter,and TNM staging(all P<0.05).The MVD value of cancer tissue in NSCLC patients with AIP1 positive expression was lower than that in AIP1 negative expression patients(P<0.05).The MVD value of cancer tissue in NSCLC patients with positive expression of EDIL3 was higher than that in patients with negative expression of EDIL3(P<0.05).The 3-year overall survival(OS)rate of NSCLC patients with positive expression of EDIL3 was lower than that of patients with negative expression of EDIL3(P<0.05),and NSCLC patients with AIP1 positive expression had higher 3-year OS rate than those with AIP1 negative expression(P<0.05).Lymph node metastasis,TNM stage IIIA,and positive expression of EDIL3 were risk factors for poor prognosis in NSCLC patients(all P<0.05),and positive expression of AIP1 was protective factor(P<0.05).Conclusions The positive expression rate of AIP1 decreases and the positive expression rate of EDIL3 increases in NSCLC cancer tissues.The positive expression rates of AIP1 and EDIL3 in the cancer tissues are related to MVD,differentiation degree,lymph node metastasis,tumor diameter,and TNM staging,and are influencing factors for the poor prognosis of NSCLC.