高压氧治疗对神经病理性疼痛大鼠疼痛行为及脊髓胶质细胞活化的影响

Effect of hyperbaric oxygen therapy on pain behavior and activation of spinal glial cells in rats with neuropathic pain

目的探究高压氧(HBO)治疗对坐骨神经慢性压迫性损伤(CCI)所致神经病理性疼痛大鼠疼痛行为及脊髓胶质细胞活化的影响。方法选取36只成年雄性SD大鼠,按照随机数字表法分为假手术组(S组)、CCI组和CCI+HBO组,每组12只。CCI+HBO组大鼠于造模后第1天开始给予HBO治疗,1 h/d,连续10 d。所有大鼠在造模前1 d及造模后第1、3、7、10天检测机械反射阈值(WMT)和热反射潜伏期(TWL)。第10天HBO治疗结束后,运用免疫荧光技术和RT-qPCR技术检测各组大鼠L4~L5节段脊髓组织胶质纤维酸性蛋白(GFAP)和离子钙结合衔接分子1(Iba-1)的表达水平。结果造模后第10天,与S组比较,CCI组大鼠的MWT明显降低、TWL明显缩短,脊髓GFAP和Iba-1表达水平明显增加(P<0.05);与CCI组比较,CCI+HBO组大鼠的MWT明显增高、TWL值明显延长,脊髓GFAP和Iba1表达水平明显下降(P<0.05或P<0.01)。结论HBO治疗可减轻CCI大鼠的神经病理性疼痛,可能是通过抑制大鼠脊髓胶质细胞的活化来实现的。

Objective To investigate the effect of hyperbaric oxygen(HBO)therapy on the pain behavior and the activation of spinal glial cells in rats with neuropathic pain.Methods A total of 36 Sprague-Dawley rats were divided into sham group(S group),chronic constriction injury of sciatic nerve group(CCI group),CCI combined with hyperbaric oxygen therapy group(CCI+HBO group),according to the random number table method,with 12 rats in each group.In the CCI+HBO group,HBO therapy started from the first day after the operation,and had been performed consecutively for 10 days with one hour per day.The mechanical withdrawal threshold(MWT)and the thermal withdrawal latency(TWL)were measured at one day before CCI injury modeling and on days 1,3,7,and 10 after CCI injury modeling.After finishing the 10-day HBO therapy,the expression levels of glial fibrillary acidic protein(GFAP)and ionized calcium-binding adapter molecule 1(Iba-1)in L4-L5 spinal cord were observed by using immunofluorescence and RT-qPCR.Results On day 10 after CCI injury modeling,compared with the S group,significant decreases of MWT and TWL were observed in the CCI group,the expression levels of GFAP and Iba-1 in spinal cord were also up-regulated in the CCI group(P<0.05).Compared with the CCI group,significant increases of MWT and TWL were observed in the CCI+HBO group,the expression levels of GFAP and Iba-1 in spinal cord were also down-regulated in the CCI+HBO group(P<0.05 or P<0.01).Conclusion HBO can relieve neuropathic pain caused by CCI injury,which might be achieved by inhibiting the activation of spinal glial cells.