NLRP3在胃癌中的表达及其临床意义

Expression of NLRP3 and Its Clinical Significance in Gastric Cancer

[目的]探讨NLRP3在胃癌中的表达及与预后的相关性。[方法]纳入GEO数据库中567例胃癌患者,分析NLRP3 mRNA的表达水平。入组昆山市中医医院2014年1月至2017年8月手术的112例临床胃癌病例组织及82例癌旁组织,免疫组化法检测NLRP3蛋白表达水平。采用单因素及多因素Cox分析NLRP3蛋白及mRNA表达水平与患者预后的相关性。[结果] GEO数据库567例胃癌患者中,NLRP3 mRNA低表达104例(18.34%),高表达463例(81.66%);单因素Cox回归分析显示,TNM分期、年龄、脉管侵犯及NLRP3 mRNA水平与胃癌预后相关(P均<0.05),多因素Cox回归分析显示,低表达NLRP3 m RNA是预后的独立危险因素(HR=1.39,95%CI:1.04~1.87,P=0.028)。112例临床胃癌患者癌组织中NLPR3免疫组化评分均值(3.49±3.16)低于癌旁组织(5.54±3.51)(P<0.001),NLPR3蛋白阴性表达18例,阳性表达94例。NLRP3蛋白表达与年龄、T分期及TNM分期相关(P均<0.05)。NLRP3蛋白阴性表达者的预后明显差于阳性表达者(HR=2.34,95%CI:1.04~5.30,P=0.041)。[结论] NLRP3低表达是胃癌预后的危险因素,NLPR3蛋白阴性表达可能是胃癌不良预后的预测指标。

[Objective] To investigate the expression of NLRP3 and its correlation with prognosis in gastric cancer.[Methods] The data of NLRP3 mRNA expression in 567 gastric cancer patients were obtained from GEO database.A total of 112 cancer tissue and 82 pericancerous normal tissue samples were collected from gastric cancer patients who received surgical treatment in Kunshan Hospital from January 2014 to August 2017,and the expression level of NLRP3 protein was detected by immunohistochemistry.The correlation between NLRP3 protein and mRNA expression levels and patient prognosis was analyzed by univariate and multivariate Cox analysis.[Results]Among the 567 gastric cancer patients from the GEO database,104 cases(18.34%) had low expression of NLRP3 mRNA and 463 cases(81.66%) had high expression.Univariate Cox regression analysis showed that TNM stage,age,lympho-vascular invasion,and NLRP3 mRNA level were correlated with the prognosis of gastric cancer patients(all P<0.05).Multivariate Cox regression analysis showed that low expression of NLRP3 mRNA was an independent risk factor for prognosis(HR=1.39,95%CI:1.04~1.87,P=0.028).Among 112 clinical gastric cancer patients,the mean immunohistochemical score of NLPR3 in cancer tissues was lower than that in pericancerous tissues(3.49±3.16 vs 5.54±3.51,P<0.001).There were 18 cases with negative expression of NLPR3protein and 94 cases with positive expression.The expression of NLRP3 protein was correlated with age,T stage,and TNM stage(all P<0.05).The prognosis of patients with negative NLRP3protein expression was significantly worse than that of positive expression patients(HR =2.34,95%CI:1.04~5.30,P=0.041).[Conclusion] Low expression of NLRP3 is a risk factor for the poor prognosis of gastric cancer patients,which may be used as predictive marker for prognosis of gastric cancer.