陈皮酵素对小鼠酒精性肝损伤及肠道菌群的调节作用

Regulatory effect of Pericarpium citri reticulatae fermentation liquor on alcoholic liver disease and gut microbiota in mice

目的探讨陈皮酵素(Pericarpium citri reticulatae fermentation liquor,PF)对小鼠酒精性肝损伤和肠道的调节作用。方法将40只C57BL/6雄性小鼠随机分为正常组、模型组、阳性药物组[100mg/(kg·d)水飞蓟宾(silibinin,Sly)]和陈皮酵素组[500 mg/(kg·d)PF],每组10只,持续给药干预2周。通过分子生物学检测生物化学和病理学指标;16S rDNA高通量测序技术检测分析粪便微生物群。结果组织切片结果显示陈皮酵素可以显著改善酒精性肝损伤小鼠的肝脏和回肠组织损伤,减少酒精诱导的肝脏脂质积累。此外,陈皮酵素显著降低肝脏组织促炎因子[白细胞介素-1β(interleukin-1β,IL-1β)、白细胞介素-6(interleukin-6,IL-6)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)]的水平,缓解酒精引起的氧化应激。同时,陈皮酵素可修复酒精引起的肠道菌群紊乱,维持肠道菌群数量,修复肠道黏膜屏障损伤。结论陈皮酵素可改善酒精性肝损伤,其机制与抗氧化、抗炎和调节肠道菌群紊乱有关,值得推广应用与开发。

Objective To investigate the regulatory effects of Pericarpium citri reticulatae fermentation liquor(PF)on alcoholic liver disease and intestinal tract in mice.Methods Forty male C57BL/6 mice were randomly divided into 4 groups,including a normal group,model group,positive drug group[100 mg/(kg·d)silibinin(Sly)],and PF group[500 mg/(kg·d)PF],with 10 mice in each group,continuous medication intervention for 2 weeks.Biochemical and pathological indicators were assessed using molecular biology techniques.Fecal microbiota was analyzed using 16S rDNA high-throughput technology.Results Histological analysis revealed that PF significantly ameliorated alcohol-induced liver and ileum tissue damage in mice,while reducing alcohol-induced liver lipid accumulation.Furthermore,PF effectively reduced pro-inflammatory factors levels(IL-1β,IL-6,TNF-α)in liver tissue and alleviated alcohol-induced oxidative stress.Additionally,PF restored alcohol-induced intestinal flora imbalance by maintaining the abundance of intestinal flora and repairing intestinal mucosal barrier damage.Conclusion These findings suggest that PF can effectively ameliorate alcoholic liver disease by exerting antioxidative and anti-inflammatory effects,as well as regulating intestinal flora disorder.These findings highlight the potential of PF for widespread application and further development.