便携式光谱仪检测白酒中乙偶姻含量

Determination of acetoin in Baijiu by portable spectrometer

目的建立一种便携式光谱仪快速检测白酒中乙偶姻含量的分析方法。方法首先利用AS7341光谱传感器搭建便携式光谱仪,结合肌酸比色法,构建一种检测白酒中乙偶姻含量的即时检测方法。接下来对肌酸比色法反应的温度和时间条件进行优化,反应结果与酶标仪进行对比。最后通过检测7种白酒样本中乙偶姻的含量,来评价本装置的准确性和实用性。结果优化后肌酸比色法的最佳反应条件为40℃反应30min,利用该装置检测到乙偶姻在510nm波长下吸光度与其浓度呈正相关关系。乙偶姻的线性检测范围为0.25~20.00mg/L,检出限为0.049mg/L。该装置检测结果与酶标仪检测结果具有很好的一致性,相关系数为0.9899。最后利用该装置对7种白酒样本中乙偶姻含量进行检测,加标回收率为96.90%~103.32%。结论该装置具有准确、快速、便捷等特点,适用于白酒中乙偶姻含量的即时检测。

Objective To establish a rapid and simple method for determining acetoin in Baijiu by a portable spectrometer.Methods A portable spectrometer was built with AS7341 spectral sensor and creatine colorimetry was used to establish a real-time method for the determination of acetoin in liquor.The temperature and time conditions of creatine colorimetric reaction were optimized,and the reaction results were compared with the enzyme-labeler.The accuracy and practicability of the device were evaluated by detecting the content of acetoin in 7 kinds of liquor samples.Results The optimal reaction conditions of creatine colorimetric assay were 40℃ for 30 minutes.The device had a positive correlation between the concentration of acetoin and absorbance at 510 nm wavelength.The linear detection range was 0.25-20.00 mg/L,and the limit of detection was 0.049 mg/L.The detection results of the device were in good agreement with those of enzyme-linked immunosorbent assay(ELISA),with a correlation coefficient of 0.9899.The recovery rates of the device for 7 kinds of Baijiu samples were between 96.90% and 103.32%.Conclusion The device is accurate,rapid,and convenient,making it suitable for on-site detection of acetoin in Baijiu.