养阴熄风通络方及其核心对药预防乌头碱致大鼠室性期前收缩的效应研究

The Effect of Yangyin Xifeng Tongluo Formula(养阴熄风通络方)and Its Core Herb-Pairs on Prevention of Aconitine-Induced Ventricular Precontraction in Rats

目的 观察养阴熄风通络方及其核心对药预防室性期前收缩(PVC)的效应。方法 实验一:40只SD大鼠随机分为模型组、养阴熄风通络方组、核心对药中剂量组、胺碘酮组,每组10只。模型组大鼠给予纯水10 ml/(kg·d)灌胃,养阴熄风通络方组大鼠给予养阴熄风通络方颗粒1.125 g/(kg·d)灌胃,核心对药中剂量组大鼠给予核心对药颗粒0.585 g/(kg·d)灌胃,胺碘酮组大鼠给予盐酸胺碘酮片18 mg/(kg·d)灌胃。各组大鼠均每日灌胃1次,连续14天后,采用大鼠尾静脉注射乌头碱25μg/kg建立PVC模型,对比各组大鼠PVC、室性心动过速(VT)、心室颤动(VF)发生率及死亡率,PVC出现时间、持续时间。实验二:60只SD大鼠随机均分为模型组、胺碘酮组及核心对药低、中、高剂量组,每组12只。模型组大鼠给予纯水10 ml/(kg·d)灌胃,核心对药低、中、高剂量组分别给予核心对药颗粒0.2925、0.585、1.17g/(kg·d)灌胃,胺碘酮组大鼠给予盐酸胺碘酮片18 mg/(kg·d)灌胃,均每日灌胃1次。14天后,尾静脉注射乌头碱25μg/kg,观察各组大鼠PVC、VT、VF发生率及死亡率,PVC出现时间、持续时间。结果 实验一:与模型组比较,养阴熄风通络方组、核心对药中剂量组、胺碘酮组大鼠VT、VF发生率及死亡率均显著降低,PVC发生率差异无统计学意义(P>0.05),PVC出现时间推迟、持续时间缩短(P<0.05或P<0.01),而各药物干预组组间比较差异均无统计学意义(P>0.05)。实验二:与模型组比较,核心对药低、中、高剂量组及胺碘酮组大鼠VT、VF发生率及死亡率均降低,PVC发生率差异无统计学意义(P>0.05),PVC出现时间推迟,持续时间缩短(P<0.05或P<0.01),而各药物干预组组间两两比较差异均无统计学意义(P>0.05)。结论 养阴熄风通络方及其核心对药均可推迟乌头碱诱导的大鼠PVC发生时间、缩短持续时间。

Objective To observe the effect of Yangyin Xifeng Tongluo Formula(养阴熄风通络方)and its core herbs combination on prevention of ventricular precontraction(PVC).Methods Experiment 1:Forty SD rats were randomly divided into model group,formula group,core herb-pairs medium-dose group and amiodarone group,with 10 rats in each group.Rats in the model group were given 10 ml/(kg·d)of pure water by gavage,rats in the formula group were given 1.125 g/(kg·d)of Yangyin Xifeng Tongluo Granules(养阴熄风通络方颗粒)by gavage,rats in the core herb-pairs medium-dose group were given 0.585 g/(kg·d)of granules of core herb-pairs by gavage,and rats in the amiodarone group were given 18 mg/(kg·d)of amiodarone hydrochloride tablets by gavage.The rats in each group were gavaged once a day for 14 consecutive days,and then a PVC model was established using rat tail vein injection of aconitine 25μg/kg to compare the mortality and incidence of PVC,ventricular tachycardia(VT)and ventricular fibrillation(VF),the time of the appearance of PVC,and the duration of PVC in the rats in each group.Experiment 2:Sixty SD rats were randomly divided into model group,the amiodarone group,and the core herb-pairs of low,medium and high dose groups,12 rats in each group.Rats in the model group were gavaged with 10 ml/(kg·d)of purified water,rats in the low,medium,and high dose groups were gavaged with 0.2925,0.585,and 1.17 g/(kg·d)of the core herb-pairs granules respectively,and rats in the amiodarone group were gavaged with 18 mg/(kg·d)of amiodarone hydrochloride tablets.All of them were gavaged once a day.After 14 days,rats in each group were injected intravenously into the tail vein with aconitine 25μg/kg,and then the rats in each group were observed to show the mortality and incidence of PVC,VT and VF,and the time of appearance and duration of PVC.Results Experiment 1:Compared with the model group,The difference in the incidence of PVC in rats of all groups was not statistically signi-ficant(P>0.05),and the mortality and incidence of VT and VF in rats in the formula group,the core herb-pairs medium-dose group,and the amiodarone group significantly reduced,with delayed time of the occurrence of PVC and shorter duration of the PVC(P<0.05 or P<0.01)as compared with the model group,whereas the difference between each group with medication intervention was not statistically significant(P>0.05).Experiment 2:There was no statistically significant difference in the incidence of PVC in all groups(P>0.05),and the mortality and incidence of VT and VF reduced in the core herb-pairs of low-,medium-,and high-dose groups and in the amiodarone group,and the appearance of PVC delayed and its duration shortened(P<0.05 or P<0.01)as compared with the control group,whereas the differences were not statistically significant in the comparison among groups with medication(P>0.05).Conclusion Both Yangyin Xifeng Tongluo Formula and its core herb-pairs could delay the time of occurrence and shorten the duration of PVC.