四生降脂疏肝汤诱导LXRα-ABCA1信号通路减少非酒精性脂肪性肝病大鼠的脂质沉积

Effect of Sisheng Jiangzhi Shugan Tang in Lowering Lipid Deposition in Rats with Non-Alcoholic Fatty Liver Disease by Inducing LXRI-ABCA1 Signaling Pathway

目的通过非酒精性脂肪肝大鼠模型探究四生降脂疏肝汤改善肝脏脂质沉积的作用机制,为四生降脂疏肝汤治疗NAFLD提供实验依据。方法使用高脂饲料构建NAFLD大鼠模型,实验分为正常组、模型组、中药高剂量组、中药低剂量组,HE染色观察肝脏的油脂蓄积情况,并测定血清中TG、TC、LDL-C、HDL-C、TNF-α、IL-13含量。使用Western blotting检测四生降脂疏肝汤对LXRα和ABCA1蛋白影响。结果大体及HE染色观察肝脏显示四生降脂疏肝汤显著降低了大鼠肝脏的脂质蓄积,抑制TNF-α分泌(P<0.05)、促进IL-13分泌(P<0.01),并降低大鼠血清中LDL-C的含量(P<0.05)。四生降脂疏肝汤增加了LXRα和ABCA1蛋白表达水平(P<0.01)。结论四生降脂疏肝汤可显著改善大鼠肝脏脂质蓄积,激活LXRα-ABCA1信号通路促进脂质外排可能是四生降脂疏肝汤缓解NAFLD的重要机制。

Objective To explore the mechanism of Sisheng Jiangzhi Shugan Tang in improving lipid deposition in the liver through rat models with non-alcoholic fatty liver disease(NAFLD)and provide experimental evidence for the treatment of NAFLD with Sisheng Jiangzhi Shugan Tang.Methods A high-fat diet was used to construct rat models with NAFLD,which were divided into a normal group,a model group,a high-dose group of traditional Chinese medicine(TCM),and a low-dose group of TCM.Hemotoxylin-eosin(HE)staining was used to observe the accumulation of oil in the liver,and serum content of TG,TC,LDL-C,HDL-C,TNF-α,and IL-13 was measured.Western blotting was used to detect the effect of Sisheng Jiangzhi Shugan Tang on LXRαTand ABCA1 protein.Results The results of gross and HE staining observation of the liver showed that Sisheng Jiangzhi Shugan Tang significantly reduced lipid accumulation in rat liver,inhibited TNF-αnsecretion(P<0.05),promoted IL-13 secretion(P<0.01),and lowered LDL-C content in rat serum(P<0.05).Sisheng Jiangzhi Shugan Tang increased the expression level of LXRαXand ABCA1 protein(P<0.01).Conclusion Sisheng Jiangzhi Shugan Tang can significantly improve lipid accumulation in rat liver,and the activation of the LXRαXABCA1 signaling pathway to promote lipid efflux may be an important mechanism by which Sisheng Jiangzhi Shugan Tang alleviates NAFLD.