褐飞虱和白背飞虱混合饲养中的共生细菌转移

Symbiotic bacterial transfer in a mixed culture of Nilaparvata lugens and Sogatella furcifera(Hemiptera:Delphacidae)

【目的】探究褐飞虱Nilaparvata lugens和白背飞虱Sogatella furcifera的种间互作是否会导致其共生细菌的变化。【方法】分别对褐飞虱和白背飞虱进行单独饲养或混合饲养,并基于16S rRNA基因测序,分析不同饲养褐飞虱和白背飞虱种群共生细菌的多样性。【结果】混合饲养的与单独饲养的相比,褐飞虱和白背飞虱的共生菌群落结构均发生明显变化且具有趋同现象;混合饲养的褐飞虱共生细菌多样性比单独饲养的显著增加;褐飞虱中的133个扩增子序列变异(amplicon sequence variants,ASVs)和白背飞虱中的143个ASVs被定义为潜在的水平转移物种。【结论】褐飞虱和白背飞虱的种间互作改变了其共生细菌群落,研究结果为深入研究基于共生细菌的稻飞虱种间互作提供了新视角。

【Aim】To investigate whether the interspecific interaction of Nilaparvata lugens and Sogatella furcifera leads to changes in their symbiotic bacteria.【Methods】N.lugens and S.furcifera were reared in either pure-or mixed-species cultures,and the diversity of the symbiotic bacteria of these populations was analyzed by 16S rRNA gene sequencing.【Results】The structure of the symbiotic bacterial communities of both N.lugens and S.furcifera in mixed-species culture changed significantly as compared with that of both N.lugens and S.furcifera in pure-species culture,and the symbiotic bacterial community structural convergence of N.lugens and S.furcifera in mixed-species culture was observed.The diversity of the symbiotic bacteria of N.lugens in mixed-species culture was significantly enhanced as compared with that in pure-species culture.A total of 133 amplicon sequence variants(ASVs)in N.lugens and 143 ASVs in S.furcifera were defined as potential horizontally transferred species.【Conclusion】Interspecific interaction between N.lugens and S.furcifera alters the symbiotic bacterial communities of the hosts,providing a new perspective of symbiotic bacteria for studying the interspecific interaction between planthoppers.