保健食品中7种烟酰胺类化合物含量测定

Determination of seven nicotinamide compounds in health products

目的:构建一种超高效液相色谱—串联质谱法(UPLC-MS/MS)同时测定保健食品中7种烟酰胺类化合物含量的方法。方法:样品用10%甲醇水溶解,通过超声提取,以10 mmol/L乙酸铵—乙腈作为梯度洗脱的流动相,采用电喷雾离子源(ESI),正负离子模式扫描,多反应监测(MRM)模式对7种烟酰胺类化合物进行监测。结果:7种烟酰胺类化合物在各质量浓度范围内呈良好线性,相关系数均>0.996,检出限(LOD)为0.075~0.600 mg/kg,回收率为84.6%~108.6%,相对标准偏差为2.1%~8.7%(n=6)。结论:该方法操作简单、快速、高效,回收率高,精密度良好,可用于保健食品中烟酰胺等类似物的定性定量分析。

Objective:This study aimed to establish an ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method for simultaneous determination of 7 nicotinamide compounds in health food.Methods:The samples were dissolved in 10%methanol water,extracted by ultrasonic,and 10 mmol/L ammonium acetate-acetonitrile was used as the mobile phase of gradient elution.The seven nicotinamide compounds were monitored by ESI,positive and negative ion scanning mode,and multiple reaction monitoring(MRM)mode.Results:The 7 nicotinamide compounds showed good linearity in the mass concentration range,and the correlation coefficients were greater than 0.996.The limit of detection(LOD)was 0.075~0.600 mg/kg,the recovery was 84.6%~108.6% with the relative standard deviation of 2.1%~8.7%(n=6).Conclusion:The method has the advantages of simple operation,high speed,high efficiency,high recovery rateand good precision,and can be used for qualitative and quantitative analysis of nicotinamide and other analogues in health care foods.