烟酰胺调控大豆幼苗耐盐碱性的生理机制研究

Physiological mechanism of nicotinamide regulating salt and alkaline tolerance in soybean seedlings

为研究烟酰胺对大豆幼苗耐盐碱性的影响及其生理机制,本研究以大豆品种合丰50为试验材料,通过对盐碱胁迫下大豆幼苗喷施不同浓度梯度的烟酰胺进行处理,分析了不同处理下大豆幼苗的生长指标和生理指标以及烟酰胺酶基因(NIC1)的表达量。结果表明,一定浓度的烟酰胺处理可以提高盐碱胁迫下大豆幼苗的生长指标株高、根长和生物量,同时提高叶片中生理指标渗透调节物质的含量、抗氧化酶活性和光合色素含量,本试验条件下,烟酰胺溶液质量浓度以50 mg/L处理时效果最佳。烟酰胺处理后,大豆叶片中NIC1基因的表达量有所上调,说明烟酰胺酶基因的上调表达能够提高大豆的耐盐碱能力。研究结果为烟酰胺调控大豆幼苗耐盐碱性的生理机制研究提供参考。

In order to explore the effects of nicotinamide on salt and alkaline tolerance of soybean seedlings and its physiological mechanism,this paper used soybean Hefeng 50 as experimental material to analyze the growth indicators and physiological indicators of soybean seedlings under different concentrations of nicotinamide sprayed under salt and alkali stress,as well as the expression level of nicotinamide enzyme genes(NIC1) under different treatments.The results showed that a certain concentration of niacinamide treatment could increase the plant height,root length,and biomass of soybean seedlings under salt alkali stress,while also increasing the content of osmoregulatory substances,antioxidant enzyme activity,and photosynthetic pigment content in leaves.Under the experimental conditions,the best effect was achieved when the mass concentration of niacinamide solution was 50 mg/L.After nicotinamide treatment,the expression level of NIC1 gene in soybean leaves was upregulated,indicating that the upregulation of nicotinamide enzyme gene could improve the salt alkali tolerance of soybean.The research results provided a reference for the physiological mechanism of nicotinamide regulating salt and alkaline tolerance in soybean seedlings.