水稻矮秆小粒突变体dsg-j的鉴定及基因定位

Identification and Gene Mapping of the Dwarf Small-Grain Mutant dsg-j in Rice

水稻是我国最重要的粮食作物之一,株高和粒型是决定水稻产量的重要因素。通过挖掘水稻株高和粒型调控基因,阐明株高和粒型的调控机制对提高水稻产量的重要意义。在水稻品种吉大718的EMS诱变突变体后代中,筛选出一株矮秆小粒突变体,命名为dwarf and small grain-JD718(dsg-j)。与野生型吉大718相比,突变体株高变矮,籽粒变小,千粒重下降。通过茎秆第一节间切片观察证实,突变体矮化表型是由细胞变小所致。遗传分析表明,突变体的表型由隐性单基因控制。进一步利用BSA-Seq分析技术将突变体dsg-j基因初步定位在第7染色体。本研究为dsg-j基因的克隆及功能分析奠定了基础,为进一步揭示株高和粒型调控的分子机制提供线索。

Rice is one of the most important cereal crops in China.Plant height and grain type are crucial factors determining rice yield.Exploring the genes regulating plant height and grain type is of great significance for improving rice productivity.In this study,a dwarf and small grain mutant was screened from the progeny of EMS-induced mutations in the rice variety Jida 718,and it was named dwarf and small grain-JD718(dsg-j).Compared to the wildtype,the mutant exhibited reduced plant height,smaller grains,and decreased thousand-grain weight.Observation of stem internode sections confirmed that the dwarf phenotype was caused by decreased cell size in the mutant.Genetic analysis revealed that the mutant phenotype was controlled by a single recessive gene.Further analysis using BSA-Seq technique preliminarily mapped the dsg-j gene to the seventh chromosome.This study laid the foundation for cloning and functional analysis of the dsg-j gene,providing clues for further elucidation of the molecular mechanisms underlying plant height and grain type regulation.