绒山羊同期发情处理后不同时间输精的受胎率比较

Effect of defined-time artificial insemination following estrus synchronization on conception rate in cashmere goats

为比较秋季绒山羊母羊同期发情后不同输精时间的冻配受胎率并筛选受胎率较高的输精时间,试验采用孕酮栓、孕马血清促性腺激素(PMSG)和氯前列烯醇药物(PGF 2α)组合处理辽宁绒山羊成年空怀繁殖母羊,诱发母羊集中发情和排卵。发情母羊分为5组,即对照组与试验1,2,3,4组,分别在发情0 h、6 h、12 h、18 h、24 h进行冻精配种,输精量0.25 mL。结果表明:对照组与试验1,2,3,4组母羊发情率分别为86.67%、83.33%、90.00%、86.67%、83.33%,受胎率分别为15.38%、56.00%、57.89%、36.00%、4.76%。由此确定母羊发情后的6~18 h为冻精配种较为适宜的时间范围。在筛选试验中,以母羊发情6 h为对照组,以母羊发情9 h、12 h、15 h、18 h为试验Ⅰ、Ⅱ、Ⅲ、Ⅳ组进行冻精配种。结果各组发情率与对照组相比均差异不显著(P>0.05);对照组及试验Ⅰ、Ⅱ、Ⅲ、Ⅳ组的冻配受胎率分别为59.25%、66.67%、57.69%、44.44%、32.00%,表明母羊发情后9 h为最佳冻精配种时间。

To compare the conception rate of defined–time artificial insemination with frozen-thawed semen following estrus synchronization in autumn in cashmere goats and define the optimal insemination time to achieve a higher conception rate,the progesterone suppository,pregnant horse serum gonadotropin(PMSG)and PGF 2αwere used to induce estrus and ovulation in Liaoning cashmere goats in this experinent.The does were divided into 1 control group and 4 experimental groups,which inseminated with 0.25 mL semen at 0 h,6 h,12 h,18 h and 24 h following the onset of estrus through estrus synchronization,respectively.The results showed that the estrus rates were 86.67%in the control group and 83.33%,90.00%,86.67%and 83.33%in the four experimental groups,respectively.The conception rates were 15.38%in the control group,and 56.00%,57.89%,36.00%and 4.76%in the four experimental groups,respectively,with suggesting that the optimal insemination time could be at 6-18 h following the onset of estrus.In the screening test,the does following the onset of estrus used as the control group(at 6 h)and four experimental groups(at 9 h,12 h,15 h and 18 h)for frozen semen insemination.The results showed that no significant difference in estrus rate among the control group and four experimental groups was found(P>0.05),and the conception rate was 59.25%,66.67%,57.69%,44.44%and 32.00%in the control group and in four experimental groups,respectively,suggesting that the optimal time for frozen semen insemination could be at 9 h following the onset of estrus through estrus synchronization.