漯河地区人群配对受体Siglec-5/14基因多态性与COPD疾病易感性的关联研究

Study on the association between polymorphism of paired receptor Siglec-5/14 gene and susceptibility to COPD disease in central and southern Henan

目的 分析漯河地区慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)人群Siglec-5/14基因多态性与疾病易感性的关联性。方法 采用病例-对照(COPD患者300人,健康对照者300人)研究,应用目标区域重测序结合多重PCR和高通量测序技术对两组人群Siglec-5/14等位基因进行分析,并比较各等位基因频率。结果 在男性人群中,rs883552 G/G基因型、rs7359936 C/C基因型在COPD患者中的频率高于健康对照组(OR=1.695,95%CI:1.016~2.827,P<0.05;OR=1.739,95%CI:1.047~2.890,P<0.05),吸烟人群COPD患病人群rs7359936 C/C基因型比例高于健康对照组(OR=1.821,95%CI:1.034~3.207,P<0.05)。结论 在漯河地区男性人群中,rs883552 G/G基因型、rs7359936 C/C基因型与COPD风险的增加具有相关性;在吸烟人群中,rs7359936 C/C基因型同样增加COPD患病的风险。

Objective To analyze the association between Siglec-5/14 gene polymorphism and disease susceptibility in chronic obstructive pulmonary disease(COPD) population in central and southern Henan.Methods In a casecontrol study(300 COPD patients and 300 healthy controls),the Siglec-5/14 alleles of the two groups were analyzed by target region resequencing combines multiple PCR and high-throughput sequencing techniques technology,and the frequencies of alleles were compared.Results In male population,the frequency of rs883552 G/G genotype and rs7359936 C/C genotype in COPD patients were higher than those in healthy control group(OR=1.695,95% CI:1.016~2.827,P<0.05;OR=1.739,95% CI:1.047~2.890,P<0.05),the proportion of rs7359936 C/C genotype in smokers with COPD was higher than that in healthy control group(OR=1.821,95% CI:1.034~3.207,P<0.05).Conclusion In male population in central and southern Henan,rs883552 G/G genotype and rs7359936 C/C genotype are correlated with the increased risk of COPD.Among smokers,rs7359936 C/C genotype also increases the risk of COPD.