脑络欣通抗缺血性脑卒中后脂质过氧化和对线粒体损伤的保护作用研究

Effect of Naoluoxintong on anti-lipid peroxidation injury and mitochondrial damage after ischemic stroke

目的 探究脑络欣通抗缺血性脑卒中后脂质过氧化损伤和对线粒体损伤的保护作用。方法 取SPF级雄性SD大鼠采用线栓法制备大鼠脑缺血再灌注模型,按照Zea Longa评分标准进行神经功能评分。将评分2~3分的模型动物随机分为模型组,脑络欣通低、中、高剂量组,另设正常组和假手术组。造模24 h后开始灌胃给药,早晚各一次,连续7 d,正常组和假手术组灌胃生理盐水。观察脑络欣通对大鼠生活状态、体重、神经功能评分、脑组织病理学、脂质过氧化物(LPO)含量、4-羟基烯醛(4-HNE)水平、线粒体形态结构和缺氧诱导因子1α(HIF-1α)表达的影响。结果 与模型组相比,脑络欣通可提高大鼠生活质量,增加大鼠体重,提高神经功能评分,减少LPO、4-HNE、HIF-1α的累积,并对脑组织病理学损伤和线粒体结构有一定的保护作用。结论 脑络欣通可以通过抑制缺血性脑卒中后的脂质过氧化以及保护线粒体损伤从而发挥神经保护作用。

Objective To determine the protective effect of Naoluoxintong(NLXT)on lipid peroxidation injury and mitochondrial damage after ischemic stroke.Methods SPF male SD rats were used to establish the cerebral ischemic/reperfusion model by suture method.The neurological function was evaluated according to Zea Longa standards.The model animals with scores of 2~3 were randomly divided into a model group,and NLXT low,middle,and high dose groups.A sham operation group and a normal group were also established.The rats were treated with NLXT for 7 days,meanwhile,rats in the normal and the sham-operated groups were gavaged with saline.The effect of NLXT on living condition,body weight,neurological function score,brain histopathology,lipid peroxide(LPO)content,4-hydroxyenal(4-HNE)level,mitochondrial morphology and hypoxia inducible factor-1αexpression were observed.Results Compared with the model group,NLXT improved the rat’s quality of life,increased the body weight and their neurological function,reduced the accumulation of LPO,4-HNE and HIF-1α,and protected against the pathological damage of the brain tissue and the structure of mitochondria.Conclusion NLXT plays a neuroprotective role by inhibiting lipid peroxidation and protecting mitochondrial damage after ischemic stroke.