ATP-ACLY在光线性角化病和皮肤鳞状细胞癌中的表达及意义

Expression of Adenosine-triphosphate citrate lyase in Actinic Keratosis and Cutaneous Squamous Cell Carcinoma

目的比较三磷酸腺苷-柠檬酸裂解酶(ATP-ACLY)在正常皮肤、光线性角化病(AK)和皮肤鳞状细胞癌(cSCC)皮损组织中的表达情况。方法在2020年10月—2021年6月就诊于本院的患者中,收集组织病理确诊为AK、cSCC以及正常皮肤的石蜡包埋组织(FFPE)标本各30例。用免疫组化SABC法检测ACLY在30例正常皮肤、30例AK和30例cSCC皮损组织标本中的表达情况。结果cSCC组中ACLY的阳性细胞率为(27.7±6.7)%,显著高于正常皮肤组(18.8±1.7)%和AK组(20.4±6.8)%。ACLY的阳性细胞率在正常皮肤组和AK组之间的差异无统计学意义(P>0.05)。ACLY在正常皮肤、AK和cSCC皮损组织中染色强阳性率分别为0.00%(0/30)、3.33%(1/30)和23.33%(7/30),cSCC组与正常皮肤组的差异有统计学意义(P<0.05)。结论ACLY的高表达可能与cSCC的发病机制相关。

Objective In order to compare the expression of adenosine-triphosphate citrate lyase(ACLY)in normal skin,solar keratosis(AK)and cutaneous squamous cell carcinoma(cSCC).Methods From October 2020 to June 2021,30 formalin-fixed,paraffin-embedded(FFPE)tissue specimens of AK,cSCC and normal skin were collected from patients in our hospital.The expression of ACLY in 30 cases of normal skin,30 cases of AK and 30 cases of cSCC tissues was detected by immunohistochemical strept avidin-biotin complex(SABC)method.Results The positive rate of ACLY in cSCC group was(27.7±6.7)%,which was significantly higher than that in normal skin group(18.8±1.7)%and AK group(20.4±6.8)%.There were no significant differences in ACLY positive cell rate between normal skin group and AK group.The positive rates of ACLY staining in normal skin,AK and cSCC tissues were 0.00%(0/30),3.33%(1/30)and 23.33%(7/30),respectively,and the differences between cSCC group and normal skin group were statistically significant(P<0.05).Conclusion The high expression of ACLY may be related to the pathogenesis of cSCC.