780株临床分离多重耐药菌分布及耐药性分析

Distribution and Antibiotic Resistance Analysis of 780 Clinical Isolates of Multidrug-resistant Bacteria

目的分析某院临床分离多重耐药菌的分布及耐药情况,为临床医护人员对感染性疾病防治与控制提供依据。方法选取2019年1月至2023年12月临床送检微生物标本纳入数据,分析临床分离多重耐药菌的分布及耐药性,采用WHONET 5.6软件和SPSS22.0软件,根据美国临床和实验室标准化协会(CLSI)2020年更新标准,分析细菌体外药敏实验数据。结果2019年1月至2023年12月送检的各类临床标本3918份,分离出多重耐药菌菌株780株,其中革兰阴性菌519株,占66.54%;革兰阳性菌212株,占27.18%;真菌49株,占6.28%,以呼吸道和尿液分离出标本较多。常见多重耐药菌前5位依次是大肠埃希菌、肺炎克雷伯菌、鲍曼不动杆菌、金黄色葡萄球菌和铜绿假单胞菌。结论分离多重耐药菌耐药率高于内蒙古地区甚至全国水平,需从医院层面提高重视程度,建立适合的安全管理体系,多部门合作并进行精细化管理,全面提升医院感控管理水平。

Objective To analyze the distribution and drug resistance of multi drug resistant bacteria isolated from a hospital,so as to provide basis for clinical medical staff to prevent and control infectious diseases.Methods Microbial samples from January 2019 to December 2023 were selected for clinical testing,and the distribution and resistance of clinically isolated multidrug-resistant bacteria were analyzed.WHONET 5.6 software and SPSS 22.0 software were used to analyze the in vitro drug sensitivity test data of bacteria according to the updated standards of the American Clinical and Laboratory Standards Institute(CLSI)in 2020.Results Among the 3918 clinical samples submitted for examination from January 2019 to December 2023,A totel of 780 strains of multi-drug resistant bacteria were isolated,of which 519 strains were gram-negative bacteria(66.54%)and 212 Gram-positive bacteria(27.18%)and 49 fungi(6.28%)with the largest number of respiratory tract and urine samples.The top 5 common multidrug-resistant bacteria are Escherichia coli,Klebsiella pneumoniae,Acinetobacter baumannii,Staphylococcus aureus,and Pseudomonas aeruginosa.Conclusion The resistance rate of multidrug-resistant bacteria isolated in our hospital was higher than the regional or even national level.It is necessary to increase the level of attention from the hospital level,establish a suitable safety management system,cooperate with multiple departments and carry out refined management,and comprehensively improve the hospital's infection control management level.