摘要
In a study of DNA methylation changes in melatonin-deficient rice mutants,mutant plants showed premature leaf senescence during grain-filling and reduced grain yield.Melatonin deficiency led to transcriptional reprogramming,especially of genes involved in chlorophyll and carbon metabolism,redox regulation,and transcriptional regulation,during dark-induced leaf senescence.Hypomethylation of mCG and mCHG in the melatonin-deficient rice mutants was associated with the expression change of both protein-coding genes and transposable element-related genes.Changes in gene expression and DNA methylation in the melatonin-deficient mutants were compensated by exogenous application of melatonin.A decreased S-adenosyl-L-methionine level may have contributed to the DNA methylation variations in rice mutants of melatonin deficiency under dark conditions.
基金
supported by the National Natural Science Foundation of China(32100448,32070558,32061143030,32170636)
Natural Science Foundation of Jiangsu Province(BK20210799)
Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD),the Seed Industry Revitalization Project of Jiangsu Province(JBGS[2021]009)
the Shanghai Science and Technology Agriculture Project([2022]No.1–6)
the Project of Zhongshan Biological Breeding Laboratory(BM2022008-029)。
