基于表型和分子标记分析24份巴戟天遗传多样性

Analysis of Genetic Diversity of 24 Morinda offi cinalis How.Based on Phenotypic and Molecular Markers

【目的】广东省德庆县是道地药材巴戟天的产地,该文对广东德庆不同地区的24份巴戟天进行了种质资源的遗传多样性分析。【方法】以德庆县各地收集来的24份巴戟天资源为研究对象,使用主成分分析和SRAP、CDDP两种分子标记方法对24份巴戟天材料进行遗传多样性分析。【结果】在表型分析中,基于主成分分析的聚类图发现,官圩镇和高良镇、凤村镇和高良镇分别聚类至4个类群中。此外,18对SRAP引物组合和16条CDDP引物扩增后分别获得100条和98条总条带,其中SRAP多态性条带为50条,多态性比率达50%,平均遗传相似系数(GS)为0.87,遗传相似系数变化范围为0.71~0.97;CDDP多态性条带为80条,条带之间多态性比率达79.0%,各种质间遗传系数范围为0.71~0.96,平均遗传相似系数为0.86,且当系数为0.82时,两种分子标记结果均表明高良镇、官圩镇和凤村镇的种质之间具有一定的相似性。【结论】结合表型和分子标记聚类结果,高良镇和官圩镇、高良镇和凤村镇的亲缘关系最近,SRAP和CDDP两种分子标记方法对巴戟天遗传多样性的结果具有一定的差异,而联合分析可以更好地反映巴戟天遗传多样性研究及其资源的系统发育关系。

【Objective】Deqing County,Guangdong Province is a native habitat of Morinda officinalis How.,a genuine medicinal herb in China.In the study,the genetic diversity of germplasm resources of 24 varieties of Morinda officinalis How.were analyzed.【Method】A total of 24 Morinda officinalis How.resources collected from Deqing County as research objects,and principal component analysis and molecular marker methods(SRAP and CDD)were used to analyze the genetic diversity of 24 Morinda officinalis How.materials.【Result】Phenotypic analysis showed that,in the cluster map based on principal component analysis,Guanwei Town,Gaoliang Town,Fengcun Town and Gaoliang Town were clustered in four groups.In addition,100 and 98 total bands were obtained in 18 pairs of SRAP primer combinations and 16 CDDP primers,among them,there were 50 SRAP polymorphic bands,resulting in a polymorphism ratio of 50%.The average genetic similarity coefficient(GS)was 0.87,and the GS ranged from 0.71 to 0.97.There were 80 polymorphic bands in CDDP,with a polymorphism ratio of 79.0%.The GS among germplasm resources ranged from 0.71 to 0.96,with an average(GS)of 0.86.When the coefficient was 0.82,both SRAP and CDDP molecular markers showed certain similarities among the germplasm resources of Gaoliang Town,Guanxu Town and Fengcun Town.【Conclusion】Combined with the clustering results of phenotypic and molecular marker,the genetic relationship is closest between Gaoliang Town and Guanwei Town as well as Gaoliang Town and Fengcun Town.The results of SRAP and CDDP molecular marker methods have certain differences in the genetic diversity of Morinda officinalis How.,and the combined analysis can better reflect the genetic diversity of Morinda officinalis How.and the phylogenetic relationship of Morinda officinalis How.resources.