黄芪多糖的超声波-闪式协同提取工艺优化及抗氧化活性研究

Optimization of Ultrasonic-Flash Assisted Extraction Process of Astragalus membranaceus Polysaccharide and Its Antioxidant Activity

以黄芪为原料,采用超声波-闪式协同提取黄芪多糖。以黄芪多糖得率为评价指标,采用单因素试验和正交试验,确定最佳提取工艺。结果表明,超声波提取最佳工艺条件为:料液比1∶30(g/mL),超声温度70℃,超声时间40 min,超声波功率500 W,在此条件下,黄芪多糖得率为7.11%;在对超声波提取工艺优化的基础上,协同闪式提取,最佳闪式提取工艺参数为:提取次数3次,闪提时间100 s,提取电压160 V,在此条件下,黄芪多糖得率为11.03%;体外抗氧化活性试验结果表明:当黄芪多糖质量浓度为1.0 mg/mL时,其对1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基和2,2-联氨-双-3-乙基苯并噻唑啉-6-磺酸(2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS)阳离子自由基的清除率分别为66.27%和58.27%。本研究获得了黄芪多糖的最佳提取方法,得到的黄芪多糖具有较强的抗氧化作用。该方法可为黄芪多糖的提取及开发应用提供参考。

Astragalus membranaceus polysaccharide(APS)was extracted from Astragalus membranaceus by ultrasonicflash assisted extraction.Taking the APS yield as evaluation index,the extraction condition was optimized by single factor test and orthogonal test.The result showed that the optimal ultrasonic extraction conditions were as follows:solidliquid ratio 1∶30(g/mL),ultrasonic temperature 70℃,ultrasonic time 40 min,ultrasonic power 500 W,under these conditions,the APS yield was 7.11%.Based on the optimized result of ultrasonic extraction,combined with flash extraction,the optimal flash extraction process paremeters were as folllus:extraction times 3,flash extraction time 100 s,extraction voltage 160 V.And the APS yield was 11.03%.The results of in vitro antioxidant activity experiments showed that when the mass concentration of APS was 1.0 mg/mL,its scavenging rates against 1,1-diphenyl-2-picrylhydrazyl(DPPH)free radicals and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)cation free radicals were 66.27%and 58.27%,respectively.Which obtained the optimal extraction method for APS with strong antioxidant effects.This method provides a reference for the extraction and development of Astragalus membranaceus polysaccharide.