IFN-αIL-6和IL-17检测对结核病辅助诊断价值

Evaluation of the Auxiliary Diagnostic Value of IFN-αIL-6 and IL-17 for Tuberculosis

目的:评价血清免疫细胞因子对结核分枝杆菌肺病(TB)的诊断价值。方法:回顾性选取2020年11月至2022年10月于河北省胸科医院就诊的139例疑似肺结核患者作为研究对象。按照2018年最新实施的肺结核诊断标准(WS288-2017),其中确诊结核病69例,纳入结核组,非结核病70例纳入非结核组。采用流式免疫荧光发光法检测血清中免疫相关细胞因子IL-6、IL-17和IFN-α水平,并分析三种因子检测对结核病的诊断效能。结果:结核组患者IL-6浓度和IL-17浓度显著高于非结核组,差异有统计学意义(P<0.05);结核组患者IFN-α浓度略高于非结核组,但差异无统计学意义(P>0.05);进行ROC曲线分析:IL-6检测AUC面积为0.658(P<0.001)、IL-17检测AUC面积为0.734(P<0.001)、IFN-α检测AUC面积为0.571(P<0.001)。结论:MTB感染者IL-6、IL-17水平增高,IL-6、IL-17对结核的诊断具有较好的灵敏度和特异度;IL-6、IL-17在临床辅助诊断结核病具有一定的价值。

Objective:To detect and analyze the specificity and sensitivity of serum immune cytokines in mycobacterium tuberculosis(TB)lung disease,and to evaluate its diagnostic value.Methods:A total of 139 patients with suspected pulmonary tuberculosis who were treated in Hebei Chest Hospital from Nov.2020 to Oct.2022 were retrospectively selected as the research subjects.According to the latest diagnostic criteria for tuberculosis implemented in 2018(WS288-2017),69 cases of confirmed tuberculosis were included in the tuberculosis group,and 70 cases of non-tuberculosis were included in the non-tuberculosis group.The serum levels of immune-related cytokines IL-6,IL-17,and IFN-αwere detected by flow immunofluorescence assay,and the diagnostic efficacy of the three factors in tuberculosis was analyzed.Results:The levels of IL-6 and IL-17 in patients with tuberculosis were significantly higher than those in patients without tuberculosis(P<0.05).The concentration of IFN-αin the TB group was slightly higher than that in the non-TB group,There's no statistically significant difference(P>0.05).ROC curve analysis showed that the area under the curve(AUC)of IL-6,IL-17,and IFN-αdetection was 0.658,0.734and 0.571,respectively(P<0.001).Conclusion:The detection of IL-6 and IL-17 has certain clinical value in the auxiliary diagnosis of tuberculosis.