爵床总黄酮有效部位对抗小鼠实验性骨质疏松的作用研究

Study on the Effect of Effective Parts of Total Flavonoids of Rostellularia Procumbens Against Murine Experimental Osteoporosis

文章探究了爵床总黄酮有效部位在对抗醋酸泼尼松致小鼠实验性骨质疏松方面的作用及机制。实验对象为50只小鼠,随机分为正常对照组、爵床总黄酮有效部位高、中、低剂量组,以及模型组。通过给小鼠灌胃醋酸泼尼松,诱导小鼠发生实验性骨质疏松症。每组小鼠分别灌胃相应药物,每日一次,连续4周后取血清检测生化指标,并对小鼠股骨进行骨物理和骨生化指标检测。结果显示,醋酸泼尼松模型组小鼠的股骨中骨羟脯氨酸、骨钙、骨磷含量明显降低(P<0.01),血清中血钙、血磷、碱性磷酸酶含量明显升高(P<0.01);而爵床总黄酮有效部位剂量组相对模型组,小鼠股骨骨重和骨质含量明显提高(P<0.01),且血清中碱性磷酸酶含量显著增加(P<0.05)。该结果验证了爵床总黄酮有效部位能对抗小鼠实验性骨质疏松的作用机制。

This research explores the effect and mechanism of effective parts of total flavonoids of Rostellularia procumbens against the murine experimental osteoporosis induced by prednisone acetate.The experimental subjects are 50 mice,which are randomly divided into the normal control group,the high,medium and low dose groups of effective parts of total flavonoids of Rostellularia procumbens and the model group.Through filled stomaches of mice with prednisone acetate,the mice are induced to develope the experimental osteoporosis.Each group of mice are filled stomaches respectively with corresponding medicines,once a day.After executing these operations for 4 weeks,the researchers take serum of these mice to test the biochemical indexes,and test the bone physical and biochemical indicators of thigh bone of mice.The results show that the level of bone hydroxyproline,bone calcium and bone phosphorus of the thigh bones of the prednisone acetate model mice are significantly decreased(P<0.01),and the level of blood calcium,blodd phosphorus and alkaline phosphatase in mice serum are significantly increased(P<0.01).Compared with the model group,the effective parts dose groups of total flavonoids of Rostellularia procumbens can significantly increase the thigh bone weight and its content(P<0.01),and increase the content of alkaline phosphatase in serum(P<0.05).Therefore,it is validated that the efective parts of total flavonoids of Rostellularia procumbens can effectively resist murine with experimental osteoporosis.