高羊茅赤霉素受体家族成员鉴定及其在不同逆境下的表达模式分析

Characterization and Analysis of Gibberellin Insensitive Dwarf1 Gene Family and Response to Abiotic Stresses in Tall Fescue

赤霉素受体(Gibberellin insensitive dwarf1)可感知赤霉素信号,参与调控植物的生长发育及逆境胁迫。高羊茅(Festuca arundinacea)是低养护成本的冷季型草坪草,面临多种逆境胁迫,但其赤霉素受体在逆境和发育中的作用尚未明确。本研究从高羊茅的转录组中鉴定到12个赤霉素受体基因序列,它们含有高度保守的结构域,可划分为两个进化枝,Ⅰ类为GID1L2,Ⅱ类为GID1。采用实时荧光定量PCR技术分析短期低温胁迫(0℃)、高温胁迫(38℃)、盐胁迫(120 mmol·L^(-1)NaCl)及干旱(15%聚乙二醇6000)逆境下高羊茅分蘖节和叶片中赤霉素受体基因的表达模式。结果表明,高羊茅赤霉素受体基因对各类逆境胁迫均有响应,GID1L2s在分蘖节和叶片中表达模式不同,GID1s则相似。除冷胁迫下叶片中FaGID1d表达模式独特,其余GID1s在不同逆境胁迫下表达模式一致。研究结果为进一步开展赤霉素受体基因参与逆境胁迫的功能解析提供了理论依据。

Gibberellin receptor(Gibberellin insensitive dwarf1)can sense the signal of gibberellin and participate in the regulation of plant growth and development,as well as in plant abiotic stress resistance.Tall fescue(Festuca arundinacea)is a low-maintenance cool-season turfgrass and is exposed to various stresses,but the role of its gibberellin receptor family in stress and development has not been reported yet.In this study,12 gibberellin receptor gene sequences were identified in the transcriptome of tall fescue,which contained highly conserved domains and could be divided into two clades,GID1L2(gibberellin receptor 1 like 2)for classⅠand GID1 for classⅡ.The expression patterns of these genes in crown and leaf of tall fescue under low temperature stress(0℃),high temperature stress(38℃),salt stress(120 mmol·L^(-1) NaCl)and short-term drought(15%PEG 6000)were determined by quantitative real-time polymerase chain reaction.The results showed that these genes were responsive to various abiotic stresses.The expression patterns of FaGID1L2 genes were different in crown and leaf,but FaGID1s’were similar.Except the expression pattern of FaGID1d in leaf under cold stress was unique,the expression pattern of the rest FaGID1s under cold stresses was consistent.These results provided a theoretical basis for further functional analysis of gibberellin receptor genes involved in stresses.