‘京枣39’离体多倍体诱导及其增殖、生根培养体系优化

In vitro polyploid induction and optimization of its proliferation and rooting culture system for ‘Jingzao 39'

【目的】研究秋水仙素溶液质量浓度、处理时间和预培养时间对‘京枣39’离体多倍体诱导的影响,优化枣树良种‘京枣39’的增殖和生根培养基,建立‘京枣39’的离体多倍体诱导体系,获得多倍体植株。【方法】采用完全随机区组试验设计,探索不同秋水仙素溶液质量浓度和处理时间组合对‘京枣39’不定芽再生的影响,不同预培养时间对‘京枣39’离体多倍体诱导的影响,不同生长调节剂组合对‘京枣39’增殖培养的影响,不同基本培养基和生长调节剂组合对‘京枣39’组培苗生根培养的影响。【结果】(1)当秋水仙素溶液质量浓度为80 mg/L、处理48 h时‘京枣39’刻伤叶片存活率为(50.00±7.07)%,分化系数为(2.06±0.17)。(2)最佳预培养时间为6 d,并施以80 mg/L秋水仙素浓度处理48 h,获得了纯合四倍体‘京枣39’。(3)最佳增殖培养基为MS+0.8 mg/L 6-BA+0.4 mg/L IBA+30 g/L麦芽糖,pH=5.8,平均增殖系数为(4.22±0.22)。(4)最佳生根培养基为改良1/2MS+0.8 mg/L IBA+30 g/L麦芽糖,pH=5.8,平均根长为(5.22±0.19) cm;平均生根数为(3.20±0.22)。【结论】初步建立了‘京枣39’的离体多倍体诱导体系,获得了‘京枣39’的多倍体植株,并优化了其增殖培养及生根培养方法。本研究为优良枣树品种‘京枣39’的种质创新提供了切实可行的技术方法,为多倍体诱导在枣树以及其他果树中的应用奠定了试验基础。

[Objective] This paper studies the effects of colchicine solution mass concentration,treatment time and pre-culture time on the induction of polyploidof ‘Jingzao 39' in vitro,optimizes the proliferation and rooting medium of ‘Jingzao 39',and an in vitro polyploid induction system for ‘Jingzao 39' was established and polyploid plants were obtained.[Method] Using randomized complete block design,we investigated the effects of different mass concentrations of colchicine solution and treatment time combinations on adventitious bud regeneration of ‘Jingzao 39',the effects of different pre-culture time on polyploid induction of ‘Jingzao 39' in vitro,the effects of different growth regulator combinations on the proliferation culture of ‘Jingzao 39' and the effects of different basic media and growth regulator combinations on the rooting culture of ‘Jingzao 39'.[Result](1) When the mass concentration of colchicine solution was 80 mg/L and treated for 48 h,the survival rate of ‘Jingzao 39' leaves was(50.00 ± 7.07)%,and the differentiation coefficient was(2.06 ± 0.17).(2) The best pre-culture time was 6 d,and the tetraploid‘Jingzao 39' was obtained with 80 mg/L colchicine and treatment for 48 h.(3) The optimal proliferation medium was MS + 0.8 mg/L 6-BA + 0.4 mg/L IBA + 30 g/L maltose,pH = 5.8,and the average proliferation coefficient was(4.22 ± 0.22).(4) The best rooting medium was modified 1/2MS + 0.8 mg/L IBA + 30 g/L maltose,pH = 5.8,and the average root length was(5.22 ± 0.19) cm,and the average rooting number was(3.20 ± 0.22).[Conclusion] The polyploid induction system of ‘Jingzao 39' is established preliminarily,and one polyploid ‘Jingzao 39' is obtained.The methods of its proliferation and rooting culture are optimized.It provides a feasible technical method for the germplasm innovation of fine jujube variety ‘Jingzao 39',and provides an experimental basis for the application of polyploid induction in jujube and other fruit trees.