四环素胁迫下小白菜生长对生物炭源溶解性有机物的响应

Response of Chinese cabbage growth to biochar dissolved organic matter under tetracycline stress

为了研究内源生物炭溶解性有机物(BDOM)对四环素类抗生素污染生物效应的影响,分别在水溶液和土壤介质中研究盐酸四环素(TC)对小白菜种子的萌发效应,在此基础上探讨BDOM输入后TC对小白菜种子发芽率和根伸长、芽伸长的影响.结果表明:同一污染水平下,小白菜的根伸长抑制率和芽伸长抑制率与TC质量浓度存在剂量-效应关系,TC在水溶液中对小白菜根伸长和芽伸长抑制效应高于土壤中,根伸长抑制率是评价TC生态毒性最佳指标;无论在水溶液还是土壤介质中,BDOM协同TC均对小白菜种子萌发起到一定程度的促进作用;在水溶液中,BDOM能够有效缓解TC的胁迫,促进芽的伸长,而对根伸长则起抑制效应.在土壤介质中,TC胁迫下BDOM对小白菜根伸长产生低浓度促进高浓度抑制的效应,对芽伸长作用表现为促进效应.

In order to study the influence of endogenous biochar dissolved organic matter(BDOM)on the biological effects of tetracycline antibiotic contamination,this paper investigated the germination effect of tetracycline hydrochloride(TC)on Chinese cabbage seeds in aqueous solution and soil medium,respectively,on the basis of which we explored the effects of TC on the germination rate and root elongation and shoot elongation of Chinese cabbage seeds after BDOM input.The results showed that there was a dose-effect relationship between root elongation inhibition and shoot elongation inhibition and the mass concentration of TC under the same pollution level,and that the inhibitory effect of TC on root elongation and shoot elongation was higher in aqueous solution than in soil,and that the inhibitory rate of root elongation was the best indicator to evaluate the ecotoxicity of TC;in both aqueous solution and soil medium,BDOM synergized with TC to promote the germination effect of Brassica napus seeds to a certain extent;in aqueous solution and soil medium,BDOM synergized with TC to promote the germination effect of BDOM.In both aqueous solution and soil medium,BDOM synergized with TC to promote seed germination of Chinese cabbage to a certain extent;in aqueous solution,BDOM could effectively alleviate the stress of TC and promote the elongation of shoots,while inhibiting the root elongation.In the soil medium,under TC stress,BDOM on cabbage root elongation low concentration to promote the effect of high concentration inhibition,the effect on the shoot elongation of the effect of promotion.