苦槠超微粉中没食子酸、鞣花酸含量测定方法的建立

Determination of gallic acid and ellagic acid in Castanopsis sclerophylla ultrafine powder by HPLC

目的建立检测苦槠超微粉中没食子酸、鞣花酸含量准确度较高的方法。方法采用超微打粉技术提取苦槠果实中的有效成分,并使用反相色谱柱在高效液相色谱仪上检测含量。选用乙腈以及0.1%磷酸水溶液作为梯度洗脱流动相,在270 nm波长下测定没食子酸和鞣花酸含量。结果没食子酸和鞣花酸分别在0.4158~10.3950μg/ml(r^(2)=0.9981)、0.1801~4.5020μg/ml(r^(2)=0.9998)的浓度范围内与峰面积有较好的线性关系,平均加样回收率:没食子酸为97.52%(6份样品RSD为2.10%),鞣花酸为98.36%(6份样品RSD为1.77%)。结论本方法操作简便、准确度及重复性良好,可为苦槠超微粉的质量研究提供依据。

Objective Establish a method for the determination of gallic acid and gallogen in Castanopsis sclerophylla ultrafine powder with high accuracy.Methods The effective components in Castanopsis sclerophylla were extracted by ultrafine powder technique and was detected on a high performance liquid chromatograph using a reversed-phase column.The gallic acid and gallogen contents were quantified at 270 nm using a mobile phase of acetonitrile and 0.1%phosphoric acid aqueous solution with gradient elution.Results Gallic acid in the concentration range of 0.4158~10.3950μg/ml(r^(2)=0.9981)and gallogen in the concentration range of 0.1801~4.5020μg/ml(r^(2)=0.9998)both had a good linearity with the peak area.The average recoverie of gallic acid was 97.52%,RSD of 6 samples was 2.10%.The average recoverie of gallogen was 98.36%,and RSD of 6 samples was 1.77%.Conclusion The method which can be used for the quality control ofC astanopsis sclerophylla ultrafine powder is simple,accurate and reproducible.