用ELISA法检测血清中抗TNF-α单克隆抗体药物浓度的方法开发与验证

Method development and validation for testing the concentration of anti-TNF-αmonoclonal antibody in serum based on ELISA

目的建立检测一种测定血清中抗肿瘤坏死因子α(TNF-α)单克隆抗体浓度的间接酶联免疫吸附测定(ELISA)方法。方法对TNF-α包被浓度及酶标抗体种类、存放稳定性和浓度等进行考察,确定关键试验参数。考察优化后方法的专属性、精密度与回收率、标准曲线与定量下限。结果TNF-α包被浓度为400 ng·mL^(-1),选择Sigma辣根过氧化物酶标记羊抗人免疫球蛋白G,以1∶3.0×10^(5)倍进行稀释;稀释后的酶标抗体4℃保存3 d稳定性良好。同时确认了方法专属性良好,回收率为84.00%~10^(6).82%,不同浓度样品精密度的变异系数均≤10%,方法线性良好,曲线方程为y=(-8.37×10^(3)-2.37×10^(6))/[1+(x/29.80)1.06]+2.37×10^(6)(R2=0.999),定量下限:1 ng·mL^(-1),均符合要求。结论本研究建立了一种准确、可靠的ELISA法测定血清中抗TNF-α单克隆抗体药物的浓度。

Objective To establish an indirect enzyme-linked immunosorbent assay(ELISA)method for testing the concentration of a monoclonal antibody target tumor necrosis factor-α(TNF-α)in animal serum.Methods The critical parameters of the method including coating concentration of human TNF-α,source,concentration and stability of HRP-labeled goat anti-human immunoglobulin G(IgG)were investigated.The specificity,accuracy,precision,linearity and Limited of Determination of the method were investigated.Results The critical parameters of the method were confirmed as below:TNF-αwas coated at 400 ng·mL^(-1);HRP labeled goat anti-human IgG antibody was diluted at 1∶3.0×10^(5);the diluted horseradish peroxidase labeled goat anti-human IgG antibody is well stored at 4℃for 3 days.Meanwhile the method was confirmed to have good specificity,the recovery rate ranged from 84.00%to 106.82%,the coefficient of variation of different antibody concentration levels were no more than 10%;the method had a good linearity and the standard curve was y=(-8.37×10^(3)-2.37×10^(6))/[1+(x/29.80)^(1.06)]+2.37×10^(6)(R~2=0.999);the limit of quantification was 1 ng·mL^(-1),all of which met the requirements.Conclusion A accurate and robust ELISA method was developed to test the concentration of anti-TNF-αmonoclonal antibody in serum.