摘要
为探究糖基化改性玉米醇溶蛋白作为壁材的抗氧化能力,提高藻油在贮藏期间的抗氧化性,利用改性玉米醇溶蛋白作为壁材包埋藻油,对包埋后藻油的储藏稳定性进行分析,并对其货架期进行预测。结果表明,改性玉米醇溶蛋白的最佳工艺条件为糖/蛋白质量比1∶12、反应时间30 min、超声功率400 W,在此条件下glu-zein的抗脂质过氧化能力达到最高,为72.31%。改性后的玉米醇溶蛋白抗脂质过氧化能力、1,1-二苯基-2-三硝基苯肼自由基清除能力、还原能力、羟自由基清除能力、超氧阴离子自由基清除能力等均有显著提高(P<0.05)。将藻油包埋在改性玉米醇溶蛋白中考察藻油的储藏稳定性,在加速氧化期间,其过氧化值、硫代巴比妥酸反应物值均低于裸藻油及未改性玉米醇溶蛋白包埋的藻油。60℃条件下储藏18 d后,glu-zein包埋的藻油POV较未改性玉米醇溶蛋白低3.68 mmol/kg,较裸藻油低39.14 mmol/kg;室温条件下与未包埋藻油货架期相比延长240 d;同时,改性后的玉米醇溶蛋白包埋的藻油二十二碳六烯酸保留率为96.95%。研究结果可为扩大改性玉米醇溶蛋白作为壁材的抗氧化性及递送易受氧气影响的生物活性物质应用提供理论依据。
This study aimed to evaluate the antioxidant capacity of glycosylated zein(glu-zein)as a wall material and improve the antioxidant properties of algal oil during storage.The storage stability of algal oil microencapsulated with glu-zein was analyzed and its shelf life was predicted.The optimal processing conditions that provided maximum anti-lipid peroxidation activity of glu-zein(72.31%)were obtained as 1:12,30 min and 400 W for mass ratio of glucose to zein,reaction time and ultrasonic power,respectively.The anti-lipid peroxidation capacity,1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging activity,reducing capacity,hydroxyl radical scavenging activity,and superoxide anion radical scavenging capacity of glu-zein were significantly increased over those of zein(P<0.05).During accelerated storage,the peroxide value(POV)and thiobarbituric acid reactive substances(TBARS)value of algal oil microencapsulated with glu-zein were lower than those of free algal oil and algal oil microencapsulated with zein,with a decrease in POV by 3.68 and 39.14 mmol/kg being found after 18 days of storage at 60℃,respectively.At room temperature,the shelf life of algal oil microencapsulated with glu-zein was extended by 240 days when compared with that of free algae oil.At the end of the storage period,algal oil encapsulated by glu-zein had the highest retention rate of docosahexaenoic acid(DHA)(96.95%).The results of this study provide a theoretical basis for expanding the application potential of modified zein as a wall material for the delivery of bioactive substances susceptible to oxygen.
作者
徐雪晗
张慧君
李萍
宋君宇
王屈园
王若懿
宋秘钊
XU Xuehan;ZHANG Huijun;LI Ping;SONG Junyu;WANG Quyuan;WANG Ruoyi;SONG Mizhao(Beverage Engineering Technological Research Center of Fruit-Vegetables and Coarse Cereals of Heilongjiang Province,College of Food and Bioengineering,Qiqihar University,Qiqihar 161006,China;Shanghai Duohui Network Technology Co.Ltd.,Shanghai 200082,China;College of Light-Industry and Textile,Qiqihar University,Qiqihar 161006,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2024年第12期68-77,共10页
Food Science
基金
黑龙江省自然科学基金面上项目(C201455)
黑龙江省教育厅基本科研业务费科研项目-校地合作项目(135309214)
黑龙江省省属高等学校基本科研业务费-科研创新平台项目(135509327)。
