LncRNA CUST-40243和CUST-47163在慢性苯中毒患者中的表达及其诊断价值

Expression of LncRNA CUST-40243 and CUST-47163 in patients with chronic benzene poisoning and its diagnostic significance

目的通过检测慢性苯中毒患者和健康对照人群中LncRNA CUST-40243和CUST-47163的表达水平,判断两者在慢性苯中毒中的诊断价值。方法采用实时荧光定量PCR对慢性苯中毒患者及健康对照组人群外周静脉血中LncRNA CUST-40243和CUST-47163表达水平进行检测,采用胞质分裂阻滞微核试验(CBMN)对2组人群淋巴细胞微核率进行检测,应用ROC曲线分析其诊断意义。结果慢性苯中毒组LncRNA CUST-40243表达水平高于健康对照组(Z=3.440,P<0.01);LncRNA CUST-47163表达水平低于健康对照组(Z=-6.962,P<0.01);淋巴细胞微核率明显高于对照组(Z=3.919,P<0.01)。ROC曲线分析结果显示:慢性苯中毒组lncRNA CUST-40243的曲线下面积(area under curve,AUC)为0.697,95%置信区间(confidence interval,CI)为0.590~0.805;lncRNA CUST-47163的AUC为0.898,95%CI为0.835~0.964;血浆淋巴细胞微核率的AUC为0.724,95%CI为0.624~0.823;慢性苯中毒组患者联合诊断的AUC为0.962,95%CI为0.921~0.980。结论LncRNA在慢性苯中毒患者中异常表达,联合淋巴细胞微核率检测有助于提高诊断效率,因此可作为慢性苯中毒筛检及诊断过程中的参考指标。

Objective To determine the diagnostic value of the expression levels of LncRNA CUST-40243 and CUST-47163 in chronic benzene poisoning patients and healthy controls.Methods Real-time fluorescence quantitative PCR was used to detect the expression levels of LncRNA CUST-40243 and CUST-47163 in peripheral venous blood of patients with chronic benzene poisoning and healthy controls.Cytokinesis arrest micronucleus test(CBMN)was used to detect the micronucleus rate of lymphocytes in the two groups,and ROC curve was used to analyze its diagnostic significance.Results The expression level of LncRNA CUST-40243 in chronic benzene poisoning group was higher than that in healthy control group(Z=3.440,P<0.01).The expression level of LncRNA CUST-47163 was lower than that of healthy controls(Z=-6.962,P<0.01).The micronucleus rate of lymphocytes was significantly higher than that of the control group(Z=3.919,P<0.01).ROC curve analysis showed that the AUC of LncRNA CUST-40243 in chronic benzene poisoning group was 0.697,and the 95%confidence interval(CI)was 0.590~0.805.The AUC of LncRNA CUST-47163 is 0.898,and the 95%confidence interval(CI)is 0.835~0.964.The AUC of plasma lymphocyte micronucleus rate was 0.724,and the 95%confidence interval(CI)was 0.624~0.823.The AUC and 95%confidence interval(CI)of patients with chronic benzene poisoning were 0.962 and 0.921~0.980 respectively.Conclusion LncRNA is abnormally expressed in patients with chronic benzene poisoning,and the detection of lymphocyte micronucleus rate is helpful to improve the diagnostic efficiency,so it can be used as a reference index in the screening and diagnosis of chronic benzene poisoning.