基于全基因组关联分析研究文昌鸡初生重性状相关的候选基因

Genome-wide association analysis revealed candidate genes related with birthweight traits in Wenchang chicken

为筛选文昌鸡初生重相关联的候选基因,本研究采集235只文昌鸡初生重表型值,结合全基因组重测序获得的12 085 689个高质量单核苷酸多态性位点(SNP),利用GEMMA软件进行全基因组关联分析(GWAS)。结果显示:与初生重相关的潜在SNP位点共有11个;通过基因功能注释,确定了6个可能影响初生重的候选基因,分别为cAMP响应元件结合蛋白5(CREB5)、锯齿状Notch配体1(JAG1)、蓝带WH2重复蛋白(COBL)、WD重复结构域11(WDR11)、FIG4磷酸肌酸5-磷酸酶(FIG4)和跨膜结构域蛋白(VSTM2A);进一步富集分析发现,ATP和钙离子结合、钙离子信号通路和MAPK信号通路在初生重中发挥重要作用。本研究结果为文昌鸡初生重相关性状提供了重要的候选分析标记,为进一步选育优良品种提供理论支持。

To identify candidate genes associated with the birth weight of Wenchang chickens,this study collected phenotypic data on birth weight from 235 individuals of the Wenchang chicken breed.Then,blood DNA was collected from these Wenchang chickens,and 12085689 high quality single nucleotide polymorphisms(SNPs)were obtained by whole genome resequencing.Finally,GEMMA software was used to perform genome-wide association study(GWAS)on the SNPs.The results showed that a total of 11 SNPs were found to be associated with the birth weight of the chickens.Six candidate genes(CREB5,JAG1,COBL,WDR11,FIG4,and VSTM2A)affecting the birthweight were identified by gene function annotation.The functional enrichment analysis showed that ATP/calcium binding,the calcium signaling pathway and the MAPK signaling pathway played important roles in the birthweight of the birds.The results of this study provided important candidate analytical markers for growth traits in Wenchang chickens and offered theoretical support for further superior breeding selection.