肉羊体外胚胎生产技术规模化应用中品种的影响

Influence of Meat Sheep Varieties on the Scale Application of in vitro Embryo Production Technology

旨在分析品种因素对OPU-IVEP商业化快繁效率的影响。本研究以经品种鉴定的健康8月龄至3周岁黑头萨福克、黑头杜泊、德克赛尔肉用种母羊为试验供体,在内蒙古赛诺种羊科技有限公司,采用OPU技术获取卵母细胞并进行体外胚胎工厂化生产或经腹腔镜输精和手术冲胚获得体内胚胎,进行胚胎移植,记录卵母细胞回收、胚胎发育以及移植妊娠的相关数据,进行统计分析。比较不同品种在体外胚胎规模化生产中卵母细胞回收效率、卵母细胞体外受精和胚胎发育能力、胚胎移植妊娠和产羔效率等方面的差异;同时,还比较了不同品种体内、外胚胎生产技术的应用效果。结果表明:1)3个品种间卵母细胞回收率差异极显著(P<0.01),黑头杜泊回收效率最高;2)3个品种的体外受精卵裂率无显著差异(P>0.05),但德克赛尔羊的囊胚发育率极显著高于其他两个品种(P<0.01);3)无论体内胚胎还是体外胚胎生产移植,各品种间胚胎移植受胎率和平均产羔数均无显著差异(P>0.05),且各品种内,体内胚胎和体外胚胎移植的平均受胎率和产羔率均无显著差异(P>0.05)。综上所述,在工厂化、规模化应用绵羊体外胚胎生产技术过程中,不同品种间卵泡发育、回收卵母细胞数、囊胚发育率等存在差异,平均卵泡数和平均回收卵母细胞最少的德克塞尔羊的囊胚发育率最高;移植后受胎和产羔情况在不同品种间无显著差异,体内胚胎和体外胚胎单次生产获得的后代数也无显著差异,但OPU-IVEP的供体利用率远大于MOET。

The aim of this study was to explore the impact of breed factors on the efficiency of commercial rapid propagation of OPU-IVEP.This study used healthy 8-month-old to 3-year-old black headed Suffolk,black headed Dorper,and Texel meat breeding ewes identified by breed identification as donors.OPU technology was used to obtain oocytes at Inner Mongolia Saino Sheep Technology Co.,Ltd.for in vitro embryo factory production or in vivo embryos obtained through laparoscopic insemination and surgical flushing.Embryo transfer was performed,and relevant data of oocyte recovery,embryo development,and transfer pregnancy were recorded for statistical analysis.Compare t The differences in oocyte recovery efficiency,oocyte in vitro fertilization and embryo development ability,embryo transfer pregnancy and lambing efficiency among different breeds in large-scale in vitro embryo production was compared;At the same time,the application effects of in vivo and in vitro embryo production technologies of different breeds were also compared.The results showed that:1)there was a significant difference in oocyte recovery rates among the 3 breeds(P<0.01),with the highest recovery efficiency observed in the black headed Dorper;2)There was no significant difference in the in vitro fertilization and cleavage rates among the 3 breeds(P>0.05),but the blastocyst development rate of Texel sheep was significantly higher than the other 2 breeds(P<0.01);3)There was no significant difference(P>0.05)in the conception rate and average number of lambs produced by embryo transfer between different breeds,whether in vivo or in vitro,and there was no significant difference(P>0.05)in the average conception rate and lambing rate of in vivo and in vitro embryo transfer among in different breeds.In summary,in the process of OPU-IVEP industrial and large-scale application in sheep in vitro embryo production,there are differences in the efficiency among different breeds,such as follicle development,number of retrieved oocytes,and blastocyst development rate.Texel sheep,which has the lowest average number of follicles and average number of retrieved oocytes,has the highest blastocyst development rate;The conception and lambing situation after transplantation,which directly affects the efficiency of rapid reproduction,did not show significant differences among different varieties,and there was no significant difference in the number of offspring obtained from single production of in vivo and in vitro embryos.However,the donor utilization rate of OPU-IVEP is higher than that of MOET.