响应面法优化羊肚菌菌丝体发酵培养基及培养条件

Optimization of culture medium and conditions for Morchella mycelium fermentation by response surface

为得到更多羊肚菌菌丝体,优化羊肚菌菌丝体发酵培养基及培养条件。采用单因素和响应面优化方法对羊肚菌菌丝体液体发酵培养基成分及培养条件进行优化研究。结果表明,通过单因素试验,最佳碳源选取蔗糖20 g/L;最佳氮源选取酵母膏25 g/L;最佳无机盐选取KH2PO42.0 g/L;最佳pH值7,温度28℃。通过响应面优化试验,在蔗糖25 g/L、酵母膏15 g/L、KH2PO42.5 g/L、温度24℃时,菌丝体干重达17.71 g/L,比原有培养基培养的菌丝体干重提升22%。研究为羊肚菌液体高效生产菌丝体,开发羊肚菌发酵类产品奠定基础。

In order to obtain more morchella mycelium and optimize the fermentation medium and culture conditions of morchella mycelium,single factor and response surface optimization method were used to optimize the composition and culture conditions of morchella mycelia liquid fermentation medium.The results show that sucrose is 20 g/L as the best carbon source by single factor test.Yeast extract is selected to be 25 g/L as the best nitrogen source.The optimal inorganic salt is selected as KH2PO4 of 2.0 g/L.The optimal pH value is 7,and the temperature is 28℃.According to the response surface optimization test,mycelium dry weight reaches 17.71 g/L at 25 g/L sucrose,15 g/L yeast paste,2.5 g/L KH2PO4 and 24℃,which is 22%higher than that of mycelium cultured in the original medium.The study lays a foundation for the efficient production of mycelium by morchella liquid and the development of morchella fermented products.